Sahin Zafer, Ozkaya Ahmet, Cuce Gokhan, Uckun Mirac, Yologlu Ertan
Department of Physiotherapy and Rehabilitation, Faculty of Health Sciences, Necmettin Erbakan University, Konya, Turkey.
KONÜDAM Experimental Medicine Application and Research Center, Necmettin Erbakan University, Konya, Turkey.
J Biochem Mol Toxicol. 2017 Sep;31(9). doi: 10.1002/jbt.21928. Epub 2017 May 3.
Testis tissue is prone to oxidation because its plasma membrane contains many polyunsaturated fatty acids. Naringenin is a plant-derived natural flavonoid. We investigated the possible ameliorative role of naringenin on the hydrogen peroxide (H O )-induced testicular damage in Wistar rats. Animals received 12 mg/kg H O by intraperitoneal injection, and 50 mg/kg naringenin via orogastric gavage for 4 weeks. In the H O group, the testis malondialdehyde level increased, while the amount of reduced glutathione, glutathione transferase activities, and the testis weight decreased. There were severe testicular damages in the H O group otherwise their grade were less in the naringenin + H O group. However, the serum testosterone concentrations decreased in both the H O and the naringenin + H O groups. The testicular zinc and calcium levels reduced in the H O -treated rats. In conclusion, the administration of H O caused oxidative stress in the testes and naringenin supplementation decreased the H O -induced effects, except for changes in testosterone levels.
睾丸组织容易发生氧化，因为其质膜含有许多多不饱和脂肪酸。柚皮素是一种植物来源的天然黄酮类化合物。我们研究了柚皮素对过氧化氢（H₂O₂）诱导的Wistar大鼠睾丸损伤的可能改善作用。动物通过腹腔注射接受12毫克/千克H₂O₂，并通过灌胃给予50毫克/千克柚皮素，持续4周。在H₂O₂组中，睾丸丙二醛水平升高，而还原型谷胱甘肽含量、谷胱甘肽转移酶活性和睾丸重量降低。H₂O₂组存在严重的睾丸损伤，否则在柚皮素+H₂O₂组中其损伤程度较轻。然而，H₂O₂组和柚皮素+H₂O₂组的血清睾酮浓度均降低。经H₂O₂处理的大鼠睾丸锌和钙水平降低。总之，给予H₂O₂会导致睾丸氧化应激，补充柚皮素可降低H₂O₂诱导产生的影响，但睾酮水平变化除外。
