Hinson Andrew M, Massoll Nicole A, Jolly Lee Ann, Stack Brendan C, Bodenner Donald L, Franco Aime T
Department of Otolaryngology - Head and Neck Surgery, University of Arkansas for Medical Sciences, Little Rock, Arkansas.
Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, Arkansas.
Head Neck. 2017 Aug;39(8):1639-1646. doi: 10.1002/hed.24807. Epub 2017 May 3.
The purpose of this study was to define and characterize the thyroid tumor-draining lymph nodes in genetically engineered mice harboring thyroid-specific expression of oncogenic Braf with and without Pten insufficiency.
After intratumoral injection of methylene blue, the lymphatic drainage of the thyroid gland was visualized in real time. The thyroid gland/tumor was resected en bloc with the respiratory system for histological analysis.
Although mice harboring Braf mutations were smaller in body size compared with their wild-type (WT) littermates, the size of their thyroid glands and deep cervical lymph nodes were significantly larger. Additionally, the tumor-draining lymph nodes showed increased and enlarged lymphatic sinuses that were distributed throughout the cortex and medulla. Tumor-reactive lymphadenopathy and histiocytosis, but no frank metastases, were observed in all mice harboring Braf mutations.
The tumor-draining lymph nodes undergo significant structural alterations in immunocompetent mice, and this may represent a primer for papillary thyroid carcinoma (PTC) metastasis.
本研究的目的是在甲状腺特异性表达致癌性Braf且有或无Pten功能不全的基因工程小鼠中，定义并表征甲状腺肿瘤引流淋巴结。
在肿瘤内注射亚甲蓝后，实时观察甲状腺的淋巴引流情况。将甲状腺/肿瘤与呼吸系统整块切除，进行组织学分析。
尽管携带Braf突变的小鼠体型比其野生型（WT）同窝小鼠小，但其甲状腺和颈深淋巴结的尺寸明显更大。此外，肿瘤引流淋巴结的淋巴窦增多且增大，分布于整个皮质和髓质。在所有携带Braf突变的小鼠中均观察到肿瘤反应性淋巴结病和组织细胞增多症，但无明显转移。
在免疫功能正常的小鼠中，肿瘤引流淋巴结会发生显著的结构改变，这可能是甲状腺乳头状癌（PTC）转移的前奏。
