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缺乏糖蛋白D的单纯疱疹病毒无法抑制病毒穿透：病毒特异性细胞表面受体的定量证据。

Herpes simplex viruses lacking glycoprotein D are unable to inhibit virus penetration: quantitative evidence for virus-specific cell surface receptors.

作者信息

Johnson D C, Ligas M W

机构信息

Department of Pathology, McMaster University, Hamilton, Ontario, Canada.

出版信息

J Virol. 1988 Dec;62(12):4605-12. doi: 10.1128/JVI.62.12.4605-4612.1988.

DOI:10.1128/JVI.62.12.4605-4612.1988
PMID:2846873
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253572/
Abstract

Herpes simplex virus (HSV) glycoprotein D (gD) plays an essential role in the entry of virus into cells. HSV mutants unable to express gD were constructed. The mutants can be propagated on VD60 cells, which supply the viruses with gD; however, virus particles lacking gD were produced in mutant-infected Vero cells. Virus particles with or without gD adsorbed to a large number (greater than 4 x 10(4] of sites on the cell surface; however, virions lacking gD did not enter cells. Cells pretreated with UV-inactivated virions containing gD (approximately 5 x 10(3) particles per cell) were resistant to infection with HSV type 1 (HSV-1) and HSV-2. In contrast, cells pretreated with UV-inactivated virions lacking gD could be infected with HSV-1 and HSV-2. If infectious HSV-1 was added prior to UV-inactivated virus particles containing gD, the infectious virus entered cells and replicated. Therefore, virus particles containing gD appear to block specific cell surface receptors which are very limited in number. Particles lacking gD are presumably unable to interact with these receptors, suggesting that gD is an essential receptor-binding polypeptide.

摘要

单纯疱疹病毒（HSV）糖蛋白D（gD）在病毒进入细胞的过程中起着至关重要的作用。构建了无法表达gD的HSV突变体。这些突变体可在能为病毒提供gD的VD60细胞上增殖；然而，在突变体感染的Vero细胞中产生的是缺乏gD的病毒颗粒。有或没有gD的病毒颗粒都吸附在细胞表面大量（大于4×10⁴）的位点上；然而，缺乏gD的病毒粒子并未进入细胞。用含gD的紫外线灭活病毒粒子（每个细胞约5×10³个颗粒）预处理的细胞对1型单纯疱疹病毒（HSV-1）和2型单纯疱疹病毒（HSV-2）感染具有抗性。相比之下，用缺乏gD的紫外线灭活病毒粒子预处理的细胞可被HSV-1和HSV-2感染。如果在含gD的紫外线灭活病毒粒子之前加入有感染性的HSV-1，感染性病毒会进入细胞并复制。因此，含gD的病毒粒子似乎会阻断数量非常有限的特定细胞表面受体。缺乏gD的粒子大概无法与这些受体相互作用，这表明gD是一种必需的受体结合多肽。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/effb/253572/58e67aea655e/jvirol00091-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/effb/253572/5e91277ceeb3/jvirol00091-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/effb/253572/fb2945806d80/jvirol00091-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/effb/253572/baf03a62e2b3/jvirol00091-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/effb/253572/58e67aea655e/jvirol00091-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/effb/253572/5e91277ceeb3/jvirol00091-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/effb/253572/fb2945806d80/jvirol00091-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/effb/253572/baf03a62e2b3/jvirol00091-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/effb/253572/58e67aea655e/jvirol00091-0186-a.jpg

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