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利用单克隆抗体纯化单纯疱疹病毒1型和2型的糖蛋白gD

Purification of glycoprotein gD of herpes simplex virus types 1 and 2 by use of monoclonal antibody.

作者信息

Eisenberg R J, Ponce de Leon M, Pereira L, Long D, Cohen G H

出版信息

J Virol. 1982 Mar;41(3):1099-1104. doi: 10.1128/JVI.41.3.1099-1104.1982.

DOI:10.1128/JVI.41.3.1099-1104.1982
PMID:6284965
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256850/
Abstract

Glycoproteins gD-1 and gD-2 of herpes simplex virus types 1 and 2, respectively, were purified on an immunoadsorbent consisting of the type-common monoclonal antibody HD-1 linked to Sepharose. Each glycoprotein was of sufficient purity, quantity, and biological activity to be used for immunological and biochemical studies. Each glycoprotein induced high titers of type-common monospecific neutralizing antibody in mice. Amino aicd analysis indicated that gD-1 and gD-2 had similar though not identical amino acid compositions.

摘要

单纯疱疹病毒1型和2型的糖蛋白gD-1和gD-2，分别通过与琼脂糖偶联的型共同单克隆抗体HD-1组成的免疫吸附剂进行纯化。每种糖蛋白都具有足够的纯度、数量和生物活性，可用于免疫学和生物化学研究。每种糖蛋白在小鼠体内诱导出高滴度的型共同单特异性中和抗体。氨基酸分析表明，gD-1和gD-2的氨基酸组成相似但不完全相同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f36d/256850/823d644b0221/jvirol00162-0366-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f36d/256850/823d644b0221/jvirol00162-0366-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f36d/256850/823d644b0221/jvirol00162-0366-a.jpg

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Herpes simplex virus glycoprotein D can bind to poliovirus receptor-related protein 1 or herpesvirus entry mediator, two structurally unrelated mediators of virus entry.单纯疱疹病毒糖蛋白D可与脊髓灰质炎病毒受体相关蛋白1或疱疹病毒进入介质结合，这两种病毒进入介质在结构上不相关。
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The gH-gL complex of herpes simplex virus (HSV) stimulates neutralizing antibody and protects mice against HSV type 1 challenge.单纯疱疹病毒（HSV）的糖蛋白gH-gL复合物可刺激产生中和抗体，并保护小鼠免受1型单纯疱疹病毒的攻击。
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