SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, KY16 9SS, UK.
Institute of Physics, University of Tartu, W. Ostwald St 1, Tartu, 50411, Estonia.
Sci Rep. 2017 May 3;7(1):1435. doi: 10.1038/s41598-017-01543-4.
We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.
我们展示了第一台使用传播不变、加速的艾里光束的光片显微镜，该显微镜可在单光子和双光子模式下工作。艾里光束的使用使我们能够开发出一种超紧凑、高分辨率的光片系统，而无需光束扫描。在双光子模式下，与使用标准高斯光束相比，视场增加了六倍。这种用于光片显微镜的实现为广泛的生物医学应用开辟了新的可能性，特别是在神经元过程的研究方面。
