Hosny Neveen A, Seyforth James A, Spickermann Gunnar, Mitchell Thomas J, Almada Pedro, Chesters Robert, Mitchell Scott J, Chennell George, Vernon Anthony C, Cho Kwangwook, Srivastava Deepak P, Forster Robert, Vettenburg Tom
M Squared Life, The Surrey Technology Centre, Guildford, Surrey, GU2 7YG, UK.
Basic and Clinical Neuroscience Department, King's College London, London, SE5 9NU, UK.
Biomed Opt Express. 2020 Jun 22;11(7):3927-3935. doi: 10.1364/BOE.395547. eCollection 2020 Jul 1.
We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.
我们展示了第一台平面艾里光片显微镜。荧光光片显微镜已成为以细胞或亚细胞分辨率研究大型生物样本的首选方法。传播不变的艾里光束在单光子激发下能使视野扩大十倍；然而，光片的特征不对称性限制了其在多光子激发方面的潜力。在此我们展示了如何从弯曲的传播不变艾里光束形成平面光片。由此产生的对称光片能在扩展的视野范围内均匀激发双光子荧光，无需去卷积。我们展示了对大量神经元组织进行快速双光子成像的方法。
