Arikawa Kaho, Wachi Akiko, Imura Yuri, Sutummaporn Kripitch, Kai Chieko, Park Eun-Sil, Morikawa Shigeru, Uematsu Yosuke, Yamaguchi Tomohiro, Furuya Tetsuya
Laboratory of Veterinary Microbiology, Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, 183-8509, Japan.
Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan.
Arch Virol. 2017 Aug;162(8):2421-2425. doi: 10.1007/s00705-017-3386-1. Epub 2017 May 3.
Feline morbillivirus (FeMV), a member of the family Paramyxoviridae, is an emerging virus that was discovered in 2012. Despite the importance of FeMV infection in cats because of its postulated involvement in kidney diseases, no simple serological assay has been reported in its detection. Here, FeMV phosphoprotein (P protein) was expressed and purified as a glutathione-S-transferase (GST)-fusion protein and used for an enzyme-linked immunosorbent assay (ELISA) to detect FeMV-specific antibodies. With a cutoff value determined by immunoblotting, anti-FeMV P protein was detected with this assay in 22 (22%) of the 100 cat plasma samples collected from various regions of Japan. This ELISA is useful for epidemiological and immunological studies, as well as for diagnosis of FeMV infection.
猫瘟病毒（FeMV）是副粘病毒科的成员，是2012年发现的一种新兴病毒。尽管由于推测FeMV感染与猫的肾脏疾病有关，其在猫感染中很重要，但尚未有简单的血清学检测方法用于其检测报道。在此，猫瘟病毒磷蛋白（P蛋白）被表达并纯化成为谷胱甘肽-S-转移酶（GST）融合蛋白，并用于酶联免疫吸附测定（ELISA）以检测猫瘟病毒特异性抗体。通过免疫印迹确定临界值，在从日本不同地区采集的100份猫血浆样本中，有22份（22%）通过该检测方法检测到抗猫瘟病毒P蛋白。这种ELISA方法对于流行病学和免疫学研究以及猫瘟病毒感染的诊断很有用。
