Bannert Erik, Tesch Tanja, Kluess Jeannette, Valenta Hana, Frahm Jana, Kersten Susanne, Kahlert Stefan, Renner Lydia, Rothkötter Hermann-Josef, Dänicke Sven
Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 50, 38116, Braunschweig, Germany.
Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany.
Mycotoxin Res. 2017 Aug;33(3):183-195. doi: 10.1007/s12550-017-0276-z. Epub 2017 May 3.
This study aimed to investigate a potential modulatory effect of E. coli lipopolysaccharide (LPS) on the kinetics of deoxynivalenol (DON) and zearalenone (ZEN) after pre- or post-hepatic LPS administration to unravel the putative role of the liver. Fifteen barrows were fed a diet containing mycotoxin-contaminated maize (4.59 mg DON/kg feed, 0.22 mg ZEN/kg feed) for 29 days and equipped with pre-hepatic catheters (portal vein, "po") and post-hepatic catheters (jugular vein, "ju"), facilitating simultaneous infusion of LPS ("LPS group", 7.5 μg/kg body weight) or 0.9% sterile NaCl solution (control, "CON group", equivolumar to LPS group) and blood sampling. This resulted in three infusion groups, depending on infusion site: CON-CON, CON-LPS, and LPS-CON. On day 29, pigs were fed their morning ration (700 g/pig) (-15 min), and blood samples were collected at regular intervals relative to infusion start. At 195 min, pigs were sacrificed and bile, urine, liquor, and liver samples collected. DON concentrations in jugular and portal blood decreased in both LPS-infused groups, whereas the ZEN concentrations increased, regardless of the treatment site. In liver tissue, a decrease of both toxin concentrations was observed in endotoxaemic pigs as well as a drop in hepatic conjugation, regardless of LPS entry site. In contrast to our hypothesis, DON and ZEN were not differently altered depending on the LPS-entry site. Neither the absorption nor the accumulation of DON and ZEN in different tissues differed significantly between animals which were infused with LPS via either the jugular or portal vein.
本研究旨在探讨大肠杆菌脂多糖（LPS）在肝前或肝后给予LPS后对脱氧雪腐镰刀菌烯醇（DON）和玉米赤霉烯酮（ZEN）动力学的潜在调节作用，以阐明肝脏的假定作用。15头公猪饲喂含有霉菌毒素污染玉米的日粮（4.59毫克DON/千克饲料，0.22毫克ZEN/千克饲料）29天，并安装肝前导管（门静脉，“po”）和肝后导管（颈静脉，“ju”），便于同时输注LPS（“LPS组”，7.5微克/千克体重）或0.9%无菌氯化钠溶液（对照组，“CON组”，与LPS组等体积）并采集血液样本。这产生了三个输注组，取决于输注部位：CON-CON、CON-LPS和LPS-CON。在第29天，给猪喂食早饲日粮（700克/头）（-15分钟），并在相对于输注开始的固定时间间隔采集血液样本。在195分钟时，处死猪并采集胆汁、尿液、脑脊液和肝脏样本。两个LPS输注组颈静脉和门静脉血中的DON浓度均降低，而ZEN浓度升高，与治疗部位无关。在内毒素血症猪的肝脏组织中，观察到两种毒素浓度均降低以及肝脏结合作用下降，与LPS进入部位无关。与我们的假设相反，DON和ZEN的变化并不因LPS进入部位而不同。通过颈静脉或门静脉输注LPS的动物之间，DON和ZEN在不同组织中的吸收和积累均无显著差异。
