1 Institute of Anatomy, Otto von Guericke University Magdeburg, Germany.
2 Institute of Animal Nutrition, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Braunschweig, Germany.
Innate Immun. 2019 Apr;25(3):168-175. doi: 10.1177/1753425919829552. Epub 2019 Feb 13.
Low concentration of LPS can be detected in healthy mammals without triggering systemic inflammation. Here we analysed the influence of the mycotoxin deoxynivalenol (DON) on very low LPS concentrations and the role of DON in the physiology of pigs challenged with high artificial LPS dosage mimicking septic shock. Pigs were fed for 29 d with DON-contaminated (4.59 mg/kg feed) or control feed. Samples of control animals showed 6.6 ± 13.5 pg/ml LPS in portal and 3.1 ± 7.6 pg/ml LPS in jugular serum samples. In the DON fed group, 3.4 ± 7.2 pg/ml and 0.6 ± 0.8 pg/ml were detected. The differences were statistically not significant, indicating that DON is not a trigger for enhanced LPS transfer into the blood circulation. Next, pigs were challenged with 7.5 µg LPS/kg body mass via portal or jugular route. The application route did not significantly influence the LPS concentration. We expected higher circulating LPS concentrations in the presence of DON due to the additional stress of liver metabolism and reduced liver capacity to remove LPS from circulation. This scenario is supported by tendency. In summary, we found that DON is unlikely to influence LPS transfer in the gut; DON likely reduces the capacity for LPS removal in septic shock conditions.
在健康的哺乳动物中，低浓度的 LPS 可以被检测到，而不会引发全身炎症。在这里，我们分析了霉菌毒素脱氧雪腐镰刀菌烯醇（DON）对极低 LPS 浓度的影响，以及 DON 在模拟败血症休克的高人工 LPS 剂量挑战下猪的生理学中的作用。猪用含有 DON（4.59 毫克/公斤饲料）或对照饲料喂养 29 天。对照动物的样本显示门静脉中 LPS 浓度为 6.6±13.5 pg/ml，颈静脉血清中 LPS 浓度为 3.1±7.6 pg/ml。在 DON 喂养组中，检测到 3.4±7.2 pg/ml 和 0.6±0.8 pg/ml。差异无统计学意义，表明 DON 不是增强 LPS 向血液循环转移的触发因素。接下来，猪通过门静脉或颈静脉途径接受 7.5µg LPS/kg 体重的挑战。应用途径对 LPS 浓度没有显著影响。由于肝脏代谢的额外压力和肝脏从循环中去除 LPS 的能力降低，我们预计在 DON 存在的情况下循环 LPS 浓度会更高。这种情况有趋势支持。总之，我们发现 DON 不太可能影响肠道中的 LPS 转移；DON 可能会降低败血症休克条件下 LPS 清除的能力。
