日粮脱氧雪腐镰刀菌烯醇是否会调节内毒素血症猪的急性期反应？——来自临床症状、白细胞计数和肿瘤坏死因子-α的经验教训。

Does Dietary Deoxynivalenol Modulate the Acute Phase Reaction in Endotoxaemic Pigs?--Lessons from Clinical Signs, White Blood Cell Counts, and TNF-Alpha.

作者信息

Tesch Tanja, Bannert Erik, Kluess Jeannette, Frahm Jana, Kersten Susanne, Breves Gerhard, Renner Lydia, Kahlert Stefan, Rothkötter Hermann-Josef, Dänicke Sven

机构信息

Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute of Animal Health, Bundesallee 50, 38116 Braunschweig, Germany.

Institute for Physiology, University of Veterinary Medicine, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.

出版信息

Toxins (Basel). 2015 Dec 23;8(1):3. doi: 10.3390/toxins8010003.

We studied the interaction between deoxynivalenol (DON)-feeding and a subsequent pre- and post-hepatic immune stimulus with the hypothesis that the liver differently mediates the acute phase reaction (APR) in pigs. Barrows (n = 44) were divided into a DON-(4.59 mg DON/kg feed) and a control-diet group, surgically equipped with permanent catheters pre- (V. portae hepatis) and post-hepatic (V. jugularis interna) and infused either with 0.9% NaCl or LPS (7.5 µg/kg BW). Thus, combination of diet (CON vs. DON) and infusion (CON vs. LPS, jugular vs. portal) created six groups: CON_CON(jug.)-CON(por.), CON_CON(jug.)-LPS(por.), CON_LPS(jug.)-CON(por.), DON_CON(jug.)-CON(por.), DON_CON(jug.)-LPS(por.), DON_LPS(jug.)-CON(por.). Blood samples were taken at -30, 15, 30, 45, 60, 75, 90, 120, 150, 180 min relative to infusion and analyzed for leukocytes and TNF-alpha. Concurrently, clinical signs were scored and body temperature measured during the same period. LPS as such induced a dramatic rise in TNF-alpha (p < 0.001), hyperthermia (p < 0.01), and severe leukopenia (p < 0.001). In CON-fed pigs, an earlier return to physiological base levels was observed for the clinical complex, starting at 120 min post infusionem (p < 0.05) and persisting until 180 min. DON_LPS(jug.)-CON(por.) resulted in a lower temperature rise (p = 0.08) compared to CON_LPS(jug.)-CON(por.). In conclusion, APR resulting from a post-hepatic immune stimulus was altered by chronic DON-feeding.

我们研究了脱氧雪腐镰刀菌烯醇（DON）喂养与肝前和肝后免疫刺激之间的相互作用，提出的假设是肝脏对猪急性期反应（APR）的介导作用存在差异。将44头公猪分为DON组（饲料中添加4.59 mg DON/kg）和对照组，手术植入永久性导管，分别位于肝前（肝门静脉）和肝后（颈内静脉），并分别输注0.9%氯化钠或脂多糖（LPS，7.5 μg/kg体重）。因此，饲料（CON组与DON组）和输注（CON组与LPS组、颈静脉输注与门静脉输注）的组合产生了六组：CON_CON（颈静脉）-CON（门静脉）、CON_CON（颈静脉）-LPS（门静脉）、CON_LPS（颈静脉）-CON（门静脉）、DON_CON（颈静脉）-CON（门静脉）、DON_CON（颈静脉）-LPS（门静脉）、DON_LPS（颈静脉）-CON（门静脉）。在输注后的-30、15、30、45、60、75、90、120、150、180分钟采集血样，分析白细胞和肿瘤坏死因子-α（TNF-α）。同时，在同一时期对临床症状进行评分并测量体温。LPS本身可导致TNF-α显著升高（p < 0.001）、体温过高（p < 0.01）和严重白细胞减少（p < 0.001）。在CON组喂养的猪中，观察到临床综合症状较早恢复到生理基线水平，从输注后120分钟开始（p < 0.05），并持续到180分钟。与CON_LPS（颈静脉）-CON（门静脉）组相比，DON_LPS（颈静脉）-CON（门静脉）组的体温升高较低（p = 0.08）。总之，慢性DON喂养改变了肝后免疫刺激引起的APR。