Bannert Erik, Tesch Tanja, Kluess Jeannette, Frahm Jana, Kersten Susanne, Kahlert Stefan, Renner Lydia, Rothkötter Hermann-Josef, Dänicke Sven
Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 50, Braunschweig 38116, Germany.
Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, Magdeburg 39120, Germany.
Toxins (Basel). 2015 Nov 16;7(11):4773-96. doi: 10.3390/toxins7114773.
Previous studies have shown that chronic oral deoxynivalenol (DON) exposure modulated Escherichia coli lipopolysaccharide (LPS)-induced systemic inflammation, whereby the liver was suspected to play an important role. Thus, a total of 41 barrows was fed one of two maize-based diets, either a DON-diet (4.59 mg DON/kg feed, n = 19) or a control diet (CON, n = 22). Pigs were equipped with indwelling catheters for pre- or post-hepatic (portal vs. jugular catheter) infusion of either control (0.9% NaCl) or LPS (7.5 µg/kg BW) for 1h and frequent blood sampling. This design yielded six groups: CON_CONjugular‑CONportal, CON_CONjugular‑LPSportal, CON_LPSjugular‑CONportal, DON_CONjugular‑CONportal, DON_CONjugular‑LPSportal and DON_LPSjugular‑CONportal. Blood samples were analyzed for blood gases, electrolytes, glucose, pH, lactate and red hemogram. The red hemogram and electrolytes were not affected by DON and LPS. DON-feeding solely decreased portal glucose uptake (p < 0.05). LPS-decreased partial oxygen pressure (pO₂) overall (p < 0.05), but reduced pCO₂ only in arterial blood, and DON had no effect on either. Irrespective of catheter localization, LPS decreased pH and base-excess (p < 0.01), but increased lactate and anion-gap (p < 0.01), indicating an emerging lactic acidosis. Lactic acidosis was more pronounced in the group DON_LPSjugular-CONportal than in CON-fed counterparts (p < 0.05). DON-feeding aggravated the porcine acid-base balance in response to a subsequent immunostimulus dependent on its exposure site (pre- or post-hepatic).
先前的研究表明，长期口服脱氧雪腐镰刀菌烯醇（DON）可调节大肠杆菌脂多糖（LPS）诱导的全身炎症，据此推测肝脏可能发挥重要作用。因此，总共41头公猪被饲喂两种以玉米为基础的日粮之一，即DON日粮（4.59毫克DON/千克饲料，n = 19）或对照日粮（CON，n = 22）。给猪植入留置导管，用于肝前或肝后（门静脉与颈静脉导管）输注对照剂（0.9%氯化钠）或LPS（7.5微克/千克体重）1小时，并频繁采集血样。该设计产生了六组：CON_CONjugular‑CONportal、CON_CONjugular‑LPSportal、CON_LPSjugular‑CONportal、DON_CONjugular‑CONportal、DON_CONjugular‑LPSportal和DON_LPSjugular‑CONportal。对血样进行血气、电解质、葡萄糖、pH值、乳酸和红细胞计数分析。红细胞计数和电解质不受DON和LPS影响。仅饲喂DON可降低门静脉葡萄糖摄取（p < 0.05）。LPS总体上降低了部分氧分压（pO₂）（p < 0.05），但仅在动脉血中降低了pCO₂，而DON对两者均无影响。无论导管位置如何，LPS均降低了pH值和碱剩余（p < 0.01），但增加了乳酸和阴离子间隙（p < 0.01），表明出现了乳酸酸中毒。DON_LPSjugular-CONportal组的乳酸酸中毒比CON饲喂组更明显（p < 0.05）。根据其暴露部位（肝前或肝后），饲喂DON会加重猪在随后免疫刺激下的酸碱平衡。
