Institute of Biotechnology, Vilnius University, 10257, Vilnius, Lithuania.
Faculty of Chemistry and Geosciences, Vilnius University, 03225, Vilnius, Lithuania.
Angew Chem Int Ed Engl. 2017 Jun 1;56(23):6507-6510. doi: 10.1002/anie.201701448. Epub 2017 May 4.
The HEN1 RNA 2'-O-methyltransferase plays important roles in the biogenesis of small non-coding RNAs in plants and proved a valuable tool for selective transfer of functional groups from cofactor analogues onto miRNA and siRNA duplexes in vitro. Herein, we demonstrate the versatile HEN1-mediated methylation and alkylation of small RNA strands in heteroduplexes with a range of complementary synthetic DNA oligonucleotides carrying user-defined moieties such as internal or 3'-terminal extensions or chemical reporter groups. The observed DNA-guided covalent functionalization of RNA broadens our understanding of the substrate specificity of HEN1 and paves the way for the development of novel chemo-enzymatic tools with potential applications in miRNomics, synthetic biology, and nanomedicine.
HEN1 RNA 2'-O-甲基转移酶在植物中小非编码 RNA 的生物发生中起着重要作用，并已被证明是一种在体外将功能基团从辅因子类似物选择性转移到 miRNA 和 siRNA 双链体上的有价值的工具。在此，我们证明了在具有一系列互补合成 DNA 寡核苷酸的异双链体中，小 RNA 链的多功能 HEN1 介导的甲基化和烷基化作用，这些寡核苷酸带有用户定义的部分，如内部或 3'-末端延伸或化学报告基团。观察到的 DNA 指导的 RNA 共价功能化拓宽了我们对 HEN1 和 p 的底物特异性的理解，为开发新型化学酶工具铺平了道路，这些工具具有在 miRNA 组学、合成生物学和纳米医学中的潜在应用。
