Chen Chang-Po, Jing Rui-Yan, Wickstrom Eric
School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, United States.
ACS Omega. 2017 Apr 30;2(4):1645-1652. doi: 10.1021/acsomega.6b00567. Epub 2017 Apr 26.
Infections are a devastating complication of titanium alloy orthopedic implants. Current therapies include antibiotic-impregnated bone cement and antibiotic-containing coatings. Daptomycin (DAP) () is a novel peptide antibiotic that penetrates the cell membranes of Gram-positive bacteria. Few DAP-resistant strains have appeared so far. We hypothesized that when DAP covalently bonded via a flexible, hydrophilic spacer it could prevent bacterial colonization of titanium alloy surfaces. We designed and synthesized a series of DAP conjugates for bonding to the surface of Ti6Al4V foils through tetra(ethylene glycol) spacers via thioether linkages. The stability and antimicrobial activity of the attached conjugates were evaluated using ATCC 25923. Colonization of the Ti6Al4V foils was inhibited by 72% at 8 h and 54% at 24 h. The strategy described in this report provides a new, more facile way to prepare bactericidal Ti6Al4V implants.
感染是钛合金骨科植入物的一种毁灭性并发症。目前的治疗方法包括含抗生素的骨水泥和含抗生素的涂层。达托霉素（DAP）是一种新型肽类抗生素，可穿透革兰氏阳性菌的细胞膜。到目前为止，很少出现对DAP耐药的菌株。我们推测，当DAP通过柔性亲水间隔基共价键合时，它可以防止细菌在钛合金表面定植。我们设计并合成了一系列DAP缀合物，通过硫醚键经四乙二醇间隔基与Ti6Al4V箔表面键合。使用ATCC 25923评估附着缀合物的稳定性和抗菌活性。Ti6Al4V箔的定植在8小时时被抑制72%，在24小时时被抑制54%。本报告中描述的策略提供了一种新的、更简便的方法来制备杀菌的Ti6Al4V植入物。
