Department of Periodontology Faculty of Dentistry, Universitat Internacional de Catalunya, Av. Josep Trueta s/n, Sant Cugat del Vallés, 08195, Barcelona, Spain.
Bioengineering Institute of Technology, Universitat Internacional de Catalunya, Av. Josep Trueta s/n, Sant Cugat del Vallés, 08195, Barcelona, Spain.
J Mater Sci Mater Med. 2018 Nov 3;29(11):164. doi: 10.1007/s10856-018-6168-8.
Peri-implantitis is an infectious disease that affects the supporting soft and hard tissues around dental implants and its prevalence is increasing considerably. The development of antibacterial strategies, such as titanium antibacterial-coated surfaces, may be a promising strategy to prevent the onset and progression of peri-implantitis. The aim of this study was to quantify the biofilm adhesion and bacterial cell viability over titanium disc with or without antibacterial surface treatment. Five bacterial strains were used to develop a multispecies oral biofilm. The selected species represent initial (Streptococcus oralis and Actinomyces viscosus), early (Veillonella parvula), secondary (Fusobacterium nucleatum) and late (Porphyromonas gingivalis) colonizers. Bacteria were sequentially inoculated over seven different types of titanium surfaces, combining different roughness level and antibacterial coatings: silver nanoparticles and TESPSA silanization. Biofilm formation, cellular viability and bacterial quantification over each surface were analyzed using scanning electron microscopy, confocal microscopy and real time PCR. Biofilm formation over titanium surfaces with different bacterial morphologies could be observed. TESPSA was able to significantly reduce the cellular viability when compared to all the surfaces (p < 0.05). Silver deposition on titanium surface did not show improved results in terms of biofilm adhesion and cellular viability when compared to its corresponding non-coated surface. The total amount of bacterial biofilm did not significantly differ between groups (p > 0.05). TESPSA was able to reduce biofilm adhesion and cellular viability. However, silver deposition on titanium surface seemed not to confer these antibacterial properties.
种植体周围炎是一种影响牙种植体周围支持软硬组织的感染性疾病，其患病率正在显著增加。抗菌策略的发展，如钛抗菌涂层表面，可能是预防种植体周围炎发生和进展的一种有前途的策略。本研究的目的是定量分析具有或没有抗菌表面处理的钛盘上的生物膜黏附与细菌细胞活力。使用五种细菌菌株来开发多菌种口腔生物膜。所选的菌种代表初始（链球菌和粘性放线菌）、早期（小韦荣球菌）、次级（核梭杆菌）和晚期（牙龈卟啉单胞菌）定植者。细菌依次接种在七种不同类型的钛表面上，结合不同的粗糙度水平和抗菌涂层：银纳米粒子和 TESPSA 硅烷化。使用扫描电子显微镜、共聚焦显微镜和实时 PCR 分析每种表面的生物膜形成、细胞活力和细菌定量。可以观察到不同细菌形态的钛表面上的生物膜形成。与所有表面相比，TESPSA 能够显著降低细胞活力（p<0.05）。与相应的非涂层表面相比，钛表面上的银沉积在生物膜黏附和细胞活力方面并没有显示出更好的结果。各组之间的细菌生物膜总量没有显著差异（p>0.05）。TESPSA 能够降低生物膜黏附与细胞活力。然而，钛表面上的银沉积似乎没有赋予这些抗菌特性。
