interdisciplinary Photonics Laboratories, School of Electrical and Data Engineering, University of Technology, Sydney (UTS), NSW 2007. Australia.
Analyst. 2017 May 30;142(11):1953-1961. doi: 10.1039/c7an00535k.
A smartphone fluorimeter capable of time-based fluorescence intensity measurements at various temperatures is reported. Excitation is provided by an integrated UV LED (λ = 370 nm) and detection obtained using the in-built CMOS camera. A Peltier is integrated to allow measurements of the intensity over T = 10 to 40 °C. All components are controlled using a smartphone battery powered Arduino microcontroller and a customised Android application that allows sequential fluorescence imaging and quantification every δt = 4 seconds. The temperature dependence of fluorescence intensity for four emitters (rhodamine B, rhodamine 6G, 5,10,15,20-tetraphenylporphyrin and 6-(1,4,8,11-tetraazacyclotetradecane)2-ethyl-naphthalimide) are characterised. The normalised fluorescence intensity over time of the latter chemosensor dye complex in the presence of Zn is observed to accelerate with an increasing rate constant, k = 1.94 min at T = 15 °C and k = 3.64 min at T = 30 °C, approaching a factor of ∼2 with only a change in temperature of ΔT = 15 °C. Thermally tuning these twist and bend associated rates to optimise sensor approaches and device applications is proposed.
本文报道了一种能够在不同温度下进行基于时间的荧光强度测量的智能手机荧光计。激发由集成的 UV LED（λ=370nm）提供，检测则使用内置的 CMOS 相机获取。集成了 Peltier 以允许在 T=10 到 40°C 的范围内测量强度。所有组件均由智能手机电池供电的 Arduino 微控制器和定制的 Android 应用程序控制，该应用程序允许每隔 δt=4 秒进行顺序荧光成像和定量。对四种荧光团（罗丹明 B、罗丹明 6G、5,10,15,20-四苯基卟啉和 6-（1,4,8,11-四氮杂环十四烷）2-乙基-萘酰亚胺）的荧光强度随温度的依赖性进行了表征。在 Zn 存在下，观察到后者化学传感器染料复合物的归一化荧光强度随时间的变化呈加速趋势，在 T=15°C 时速率常数 k=1.94 min，在 T=30°C 时 k=3.64 min，仅在温度变化 ΔT=15°C 时就接近 2 的倍数。提出了通过热调谐这些扭和弯曲相关速率来优化传感器方法和器件应用的方法。
