Delgado-Gardea Ma Carmen E, Tamez-Guerra Patricia, Gomez-Flores Ricardo, Mendieta-Mendoza Aurora, Zavala-Díaz de la Serna Francisco Javier, Contreras-Cordero Juan Francisco, Erosa-de la Vega Gilberto, Pérez-Recoder María Concepción, Sánchez-Ramírez Blanca, González-Horta Carmen, Infante-Ramírez Rocío
Laboratorio de Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Nuevo Campus Universitario No.1, Chihuahua 31125, Mexico.
Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Ave. Universidad s/n, San Nicolás de los Garza 66455, Mexico.
Int J Environ Res Public Health. 2017 May 5;14(5):482. doi: 10.3390/ijerph14050482.
In areas lacking potable water treatment, drinking contaminated water may represent a public health threat. In addition to enteropathogenic bacteria and parasites, fecal contamination in water environments is associated with the transmission of enteric viruses and other causal agents of infectious disease. Rotavirus and norovirus are the main enteric viral agents responsible for diarrheic outbreaks. The aim of the present study was to detect seasonal variation of rotavirus and norovirus in the surface water at Bassaseachic Falls National Park during 2013. Rivers and streams within and nearby this park were sampled once in each season during 2013. Viral concentration was carried out by a handmade filtration equipment, using a commercial electropositive membrane coupled with the virus absortion elution technique (VIRADEL©). Detection of rotavirus and norovirus was performed by SYBR Green reverse transcription-real time polymerase chain reaction (SYBR GREEN© RT-qPCR) analyses. Norovirus genogroup II was detected in samples collected in June and October 2013. In the case of rotavirus, genogroup A was detected in March and June. The presence of rotavirus and norovirus was related to viral acute diarrhea in children less than five years of age, who were inhabiting the sampled areas. This may indicates that the contaminated water was potentially a risk factor for regional diarrheic outbreaks.
在缺乏饮用水处理设施的地区，饮用受污染的水可能构成公共卫生威胁。除了肠道致病细菌和寄生虫外，水环境中的粪便污染还与肠道病毒及其他传染病病原体的传播有关。轮状病毒和诺如病毒是导致腹泻暴发的主要肠道病毒病原体。本研究的目的是检测2013年巴萨塞阿契克瀑布国家公园地表水中轮状病毒和诺如病毒的季节性变化。2013年期间，该公园内及附近的河流和溪流每个季节采样一次。采用手工过滤设备，使用商业正电膜结合病毒吸附洗脱技术（VIRADEL©）进行病毒浓缩。通过SYBR Green逆转录实时聚合酶链反应（SYBR GREEN© RT-qPCR）分析检测轮状病毒和诺如病毒。在2013年6月和10月采集的样本中检测到诺如病毒II基因组。就轮状病毒而言，在3月和6月检测到A基因组。轮状病毒和诺如病毒的存在与居住在采样地区的5岁以下儿童的病毒性急性腹泻有关。这可能表明受污染的水可能是区域腹泻暴发的一个风险因素。
