Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, 51666-16471 Tabriz, Iran; Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran.
Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471 Tabriz, Iran.
Chemosphere. 2017 Aug;181:655-665. doi: 10.1016/j.chemosphere.2017.04.142. Epub 2017 Apr 28.
Nanoparticles have potential high risks for living organisms in the environment due to their specific qualities and their easy access. In the present study, selenium nanoparticles (Se NPs) with two different coatings (l-cysteine and tannic acid) were synthesized. The characteristics of particles were analyzed using XRD, FT-IR and SEM. The impact of the nanoparticles besides Se, on the aquatic higher plant Lemna minor was evaluated and compared. Entrance of l-cysteine and tannic acid capped Se NPs in the roots of Lemna minor was proved by TEM and fluorescence microscopy. Adverse effects of mentioned NPs and differences of these effects from those by sodium selenite as the ionic form were assessed by a range of biophysicochemical tests. Altogether, the results asserted that Lemna minor was notably poisoned by both capped Se NPs and Se. Thus, growth and photosynthetic pigments were decreased while lipid peroxidation along with total phenol and flavonoid contents were raised. Eventually some changes in enzymatic activities were presented. To sum up the consequences, it can be concluded that all changes occurred due to the plant defense system especially in order to remove reactive oxygen species (ROS) and possible phytotoxicity originated by l- cysteine and tannic acid capped Se NPs in addition to Se. The influence of tannic acid capped Se NPs after sodium selenite is stronger by the means of antioxidant enzymes activity in comparison with l-cysteine capped Se NPs.
由于纳米粒子具有特殊的性质和易于进入环境的特点，因此对生物体具有潜在的高风险。在本研究中，合成了两种不同涂层（半胱氨酸和单宁酸）的硒纳米粒子（Se NPs）。使用 XRD、FT-IR 和 SEM 分析了颗粒的特性。评估并比较了纳米粒子（除 Se 之外）对水生高等植物浮萍的影响。通过 TEM 和荧光显微镜证明了半胱氨酸和单宁酸包覆的 Se NPs 进入浮萍的根部。通过一系列生物物理化学测试评估了上述 NPs 的不良影响及其与亚硒酸钠（离子形式）的这些影响的差异。总之，结果表明，两种包覆的 Se NPs 和 Se 都明显地毒害了浮萍。因此，生长和光合色素减少，而脂质过氧化以及总酚和类黄酮含量增加。最终，还出现了一些酶活性的变化。总之，可以得出结论，所有的变化都是由于植物的防御系统引起的，特别是为了清除活性氧（ROS）和半胱氨酸和单宁酸包覆的 Se NPs 以及 Se 可能引起的植物毒性。与半胱氨酸包覆的 Se NPs 相比，单宁酸包覆的 Se NPs 对亚硒酸钠的影响更强，这是通过抗氧化酶活性实现的。
