中国梅花鹿（Cervus nippon）弓形虫感染的分子检测与基因特征分析
Molecular detection and genetic characterization of Toxoplasma gondii infection in sika deer (Cervus nippon) in China.
作者信息
Cong Wei, Qin Si-Yuan, Meng Qing-Feng, Zou Feng-Cai, Qian Ai-Dong, Zhu Xing-Quan
机构信息
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China; College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province 130118, PR China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China; General Station for Surveillance of Wildlife Diseases & Wildlife Borne Diseases, State Forestry Administration (SFA), Shenyang 110034, PR China.
出版信息
Infect Genet Evol. 2016 Apr;39:9-11. doi: 10.1016/j.meegid.2016.01.005. Epub 2016 Jan 6.
The objective of the present study was to investigate the prevalence and genetic characterization of Toxoplasma gondii infection in sika deer in China. During August 2014 to November 2014, a total of 450 tissue samples coming from 150 sika deer were collected to detect the T. gondii B1 gene using a nested PCR, and the positive samples were genotyped at 11 genetic markers (SAG1, 5'- and 3'-SAG2, alternative SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and Apico) using multilocus polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. Seventeen of 150 sika deer (11.33%) were tested positive by nested PCR. Six DNA samples from the 17 positive samples were completely typed, in which 4 samples from lung tissues, and 2 from muscular tissues, were identified as ToxoDB Genotype #9 (http://toxodb.org/toxo/). The results of the present study revealed the existence of T. gondii infection in sika deer in China, which provided the information of T. gondii genetic diversity in this host species. This study also indicated that ToxoDB Genotype #9 has a wide distribution in sika deer that could be potential reservoirs for T. gondii transmission, which may pose a threat to human health.
本研究的目的是调查中国梅花鹿弓形虫感染的流行情况和基因特征。在2014年8月至2014年11月期间，共采集了来自150只梅花鹿的450份组织样本，采用巢式PCR检测弓形虫B1基因，并使用多位点聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对阳性样本的11个基因标记（SAG1、5'-和3'-SAG2、替代SAG2、SAG3、BTUB、GRA6、L358、PK1、c22-8、c29-2和Apico）进行基因分型。150只梅花鹿中有17只（11.33%）经巢式PCR检测呈阳性。对17份阳性样本中的6份DNA样本进行了完整分型，其中4份来自肺组织，2份来自肌肉组织，被鉴定为ToxoDB基因型#9（http://toxodb.org/toxo/）。本研究结果揭示了中国梅花鹿中存在弓形虫感染，提供了该宿主物种中弓形虫遗传多样性的信息。本研究还表明，ToxoDB基因型#9在梅花鹿中分布广泛，梅花鹿可能是弓形虫传播的潜在宿主，这可能对人类健康构成威胁。
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