Uhong Lü Yuhong, Liu Xiaoli, Wang Miao, Li Yuanyuan, Liu Ning, Bao Yuxin, Liu Minghao, Li Xiaoqian, Wang Yinyin, Qian Shenyan, Yue Changwu, Huang Ying
Department of Histology and Embryology, College of Basic Medicine, Zunyi Medical University, Zunyi, Guizhou, China / Central Laboratory of Third Affiliated Hospital, Zunyi Medical University, Zunyi, Guizhou, China.
Department of Pathology of Third Affiliated Hospital, Zunyi Medical University, Zunyi, Guizhou, China.
Pak J Pharm Sci. 2016 Sep;29(5 Suppl):1811-1815.
In order to obtain the natural products synthesized by the three putative xiamycin biosynthesis gene clusters which were predicted via antiSMASH during the genome mining of marine Streptomyces sp. FXJ 7.388, Streptomyces sp. FXJ 8.012, and Streptomyces olivaceus FXJ 7.023. Sixteen genes involved in xiamycin assembly, modification, and regulation with higher identity than the newest reported xiamycin biosynthetic gene cluster from marine Streptomyces sp. SCSIO 02999, Streptomyces sp. HKI0576, and Streptomyces sp. FXJ 7.388 were discovered via gene cluster comparative analysis. A ribosome engineering strategy was adopted to activate such cryptic gene clusters with different final concentrations antibiotics that act on the ribosome, and two indolosesquiterpenes were isolated from idlethaldose streptomycin-resistant Streptomyces sp. FXJ 7.388 strains. However, no such product was detected in Streptomyces sp. FXJ 8.012 and Streptomyces olivaceus FXJ 7.023 under the same treatment. This result suggested that these genes might hold the least gene content for xiamycin biosynthesis.
为了获得由三个假定的夏霉素生物合成基因簇合成的天然产物，这些基因簇是在对海洋链霉菌属FXJ 7.388、链霉菌属FXJ 8.012和橄榄色链霉菌FXJ 7.023进行基因组挖掘过程中通过antiSMASH预测得到的。通过基因簇比较分析，发现了16个参与夏霉素组装、修饰和调控的基因，其与最新报道的来自海洋链霉菌属SCSIO 02999、链霉菌属HKI0576和链霉菌属FXJ 7.388的夏霉素生物合成基因簇具有更高的同源性。采用核糖体工程策略，用作用于核糖体的不同终浓度抗生素激活这些隐秘基因簇，并从耐异戊醛链霉素的链霉菌属FXJ 7.388菌株中分离出两种吲哚倍半萜。然而，在相同处理下，在链霉菌属FXJ 8.012和橄榄色链霉菌FXJ 7.023中未检测到此类产物。这一结果表明，这些基因可能具有最少的夏霉素生物合成基因含量。
