Hao Chengcheng, Cui Yuxin, Hu M U, Zhi Xiuyi, Zhang Lijian, Li Wenbin, Wu Wei, Cheng Shan, Jiang Wen G
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing, P.R. China.
Beijing Key Laboratory of Cancer & Metastasis Research, Capital Medical University, Beijing, P.R. China.
Anticancer Res. 2017 May;37(5):2245-2254. doi: 10.21873/anticanres.11561.
Osteopontin (OPN) is known to be involved in the development of certain cancers, including non-small cell lung cancer (NSCLC). However, its role in tumour progression remains unclear. The present study investigated the expression and biological impact of the OPN variant, OPN-a in NSCLC.
OPN-a splicing variant expression in human NSCLC tissues was analyzed by real-time qPCR and immunohistochemistry (IHC), respectively. The impact of OPN-a on cellular functions of lung cancer cells was also evaluated. In addition, an in vitro model was developed for the assessment of interactions between lung cancer cells and bone tissue.
The expression of OPN-a was higher in lung cancer tissues compared to normal controls. OPN-a promoted the malignant phenotypes of A549 cells by enhancing cell-adherent abilities to bone tissues, which could be mediated by the interaction with the cell surface receptor αvβ3 integrin.
OPN-a may represent a bone metastatic factor in human lung cancer, as well as a potential therapy target.
已知骨桥蛋白（OPN）参与包括非小细胞肺癌（NSCLC）在内的某些癌症的发展。然而，其在肿瘤进展中的作用仍不清楚。本研究调查了OPN变体OPN-a在NSCLC中的表达及其生物学影响。
分别通过实时定量PCR和免疫组织化学（IHC）分析人NSCLC组织中OPN-a剪接变体的表达。还评估了OPN-a对肺癌细胞细胞功能的影响。此外，建立了体外模型以评估肺癌细胞与骨组织之间的相互作用。
与正常对照相比，肺癌组织中OPN-a的表达更高。OPN-a通过增强细胞对骨组织的粘附能力来促进A549细胞的恶性表型，这可能是由与细胞表面受体αvβ3整合素的相互作用介导的。
OPN-a可能是人类肺癌中的一种骨转移因子，也是一个潜在的治疗靶点。
