人γ-氨基丁酸A受体α亚基cDNA克隆的分离

Isolation of a cDNA clone for the alpha subunit of the human GABA-A receptor.

作者信息

Garrett K M, Duman R S, Saito N, Blume A J, Vitek M P, Tallman J F

机构信息

Department of CNS Research, Lederle Laboratories, Pearl River, NY.

出版信息

Biochem Biophys Res Commun. 1988 Oct 31;156(2):1039-45. doi: 10.1016/s0006-291x(88)80949-5.

DOI:10.1016/s0006-291x(88)80949-5

PMID:2847710

Abstract

A cDNA clone of an alpha subunit of the human GABA-A receptor has been isolated. The human clone (pCLL800) contains 1055 nucleotides in an open reading frame and 260 nucleotides in the 5' non-coding region. The 351 amino acid sequence of this human alpha subunit shows 97% homology with its bovine counterpart. Hybridization of pCLL800 to Northern blots shows a 3.9/4.3 Kb RNA doublet in human cortex, rat whole brain, cortex, hippocampus, midbrain, olfactory bulb and cerebellum. Developmental studies show that the levels of the rat alpha mRNA increase between one and three weeks of age in a manner similar to the development of the benzodiazepine binding sites.

摘要

已分离出人类γ-氨基丁酸A受体α亚基的一个cDNA克隆。该人类克隆（pCLL800）在开放阅读框中含有1055个核苷酸，在5'非编码区含有260个核苷酸。该人类α亚基的351个氨基酸序列与其牛对应物显示出97%的同源性。pCLL800与Northern印迹杂交显示，在人类皮质、大鼠全脑、皮质、海马体、中脑、嗅球和小脑中存在3.9/4.3 kb的RNA双峰。发育研究表明，大鼠α mRNA的水平在1至3周龄之间以类似于苯二氮䓬结合位点发育的方式增加。