Angkasekwinai Pornpimon
Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, 12120, Thailand.
Graduate Program, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, 12120, Thailand.
Methods Mol Biol. 2017;1585:189-199. doi: 10.1007/978-1-4939-6877-0_15.
IL-9-producing T helper cells (Th9) have recently emerged as an important T cell subset contributing to the pathogenicity of allergic diseases. Here, we describe the role of Th9 cells in allergic inflammation and provide detailed protocols to characterize IL-9-producing T cells and analyze OVA-specific IL-9 production in allergic lung diseases using a mouse model of OVA-induced chronic allergic lung inflammation.
产生白细胞介素-9的辅助性T细胞（Th9）最近已成为导致过敏性疾病致病性的重要T细胞亚群。在此，我们描述了Th9细胞在过敏性炎症中的作用，并提供详细方案，以利用卵清蛋白（OVA）诱导的慢性过敏性肺炎小鼠模型来鉴定产生白细胞介素-9的T细胞，并分析过敏性肺部疾病中OVA特异性白细胞介素-9的产生情况。
