Yui Y, Katayose Y, Shishido K
Department of Life Science, Faculty of Science, Tokyo Institute of Technology, Yokohama, Japan.
Biochim Biophys Acta. 1988 Nov 10;951(1):53-60. doi: 10.1016/0167-4781(88)90024-3.
Two linear plasmid-like DNA elements, designated pLP01 and pLP02, have been isolated from a strain of Pleurotus ostreatus, an edible basidiomycete. pLP01 (10.0 kb) and pLP02 (9.4 kb) were found in mitochondrial preparations of the fungus and appear to have 5' ends blocked by association of a protein. Proteinase K cleavability of the 5'-terminal protein of pLP01 was higher than that of pLP02, indicating that the terminal proteins of both plasmid-like elements are distinct from one another. pLP01 and pLP02 were estimated to be present to the extent of 1-2 copies each per mitochondrial genome equivalent. The two plasmid-like elements had no homology between them and also were not homologous with the mitochondrial and nuclear genomic DNAs of the fungus.
从食用担子菌平菇的一个菌株中分离出了两个线性质粒样DNA元件，分别命名为pLP01和pLP02。在该真菌的线粒体提取物中发现了pLP01（10.0 kb）和pLP02（9.4 kb），它们的5'端似乎因与一种蛋白质结合而被封闭。pLP01的5'端蛋白质对蛋白酶K的可切割性高于pLP02，这表明这两个质粒样元件的末端蛋白质彼此不同。据估计，每个线粒体基因组当量中pLP01和pLP02各有1 - 2个拷贝。这两个质粒样元件彼此之间没有同源性，也与该真菌的线粒体和核基因组DNA没有同源性。
