Wu Peng, Yao Tian, Ren Yuanhang, Ye Jinghua, Qing Yuan, Li Qiang, Gui Mingying
Yunnan Plateau Characteristic Agricultural Industry Research Institute, Yunnan Agricultural University, Kunming, China.
School of Food and Biological Engineering, Chengdu University, Chengdu, China.
Front Microbiol. 2021 Jul 5;12:583129. doi: 10.3389/fmicb.2021.583129. eCollection 2021.
The genus is a group of global ectomycorrhizal fungi. The characterizations of mitochondrial genomes have still been unknown. In the present study, the complete mitogenomes of two species, , and , were assembled and compared with other Boletales mitogenomes. Both mitogenomes comprised circular DNA molecules with sizes of 43,990 and 44,054 bp, respectively. Comparative mitogenomic analysis showed that the gene differentiated greatly between Boletales species, and this gene may be subjected to strong pressure of positive selection between some Boletales species. Several plasmid-derived genes and genes with unknown functions were detected in the two mitogenomes, which needs further analysis. The two species show a high degree of collinearity, which may represent the gene arrangement of the ancestors of ectomycorrhizal Boletales species. Frequent intron loss/gain events were detected in Boletales and basidiomycetes, and intron P717 was only detected in out of the eight Boletales mitogenomes tested. We reconstructed phylogeny of 79 basidiomycetes based on combined mitochondrial gene dataset, and obtained well-supported phylogenetic topologies. This study served as the first report on the mitogenomes of the family Pisolithaceae, which will promote the understanding of the evolution of species.
该属是一类全球分布的外生菌根真菌。其线粒体基因组的特征仍不清楚。在本研究中，组装了两种豆包菌属物种（[物种名称1]和[物种名称2]）的完整线粒体基因组，并与其他牛肝菌目线粒体基因组进行了比较。两种豆包菌属线粒体基因组均由环状DNA分子组成，大小分别为43,990和44,054 bp。线粒体基因组比较分析表明，牛肝菌目物种之间该基因差异很大，并且该基因在一些牛肝菌目物种之间可能受到强烈的正选择压力。在这两种豆包菌属线粒体基因组中检测到几个源自质粒的基因和功能未知的基因，这需要进一步分析。这两种豆包菌属物种表现出高度的共线性，这可能代表外生菌根牛肝菌目物种祖先的基因排列。在牛肝菌目和担子菌中检测到频繁的内含子丢失/获得事件，在所测试的八个牛肝菌目线粒体基因组中，仅在[具体物种]中检测到内含子P717。我们基于线粒体基因数据集重建了79种担子菌的系统发育，获得了支持度良好的系统发育拓扑结构。本研究作为豆包菌科线粒体基因组的首次报道，将促进对豆包菌属物种进化的理解。
