Da Juan, Cheng Chun-Ru, Yao Shuai, Long Hua-Li, Wang Yan-Hong, Khan Ikhlas A, Li Yi-Feng, Wang Qiu-Rong, Cai Lu-Ying, Jiang Bao-Hong, Liu Xuan, Wu Wan-Ying, Guo De-An
Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
Phytochemistry. 2015 Jun;114:146-54. doi: 10.1016/j.phytochem.2014.08.007. Epub 2014 Sep 8.
Ultra-performance liquid chromatography (UPLC) and Single Standard for Determination of Multi-Components (SSDMC) are becoming increasingly important for quality control of medicinal herbs; this approach was developed for Ganoderma lucidum. Special attention was necessary for the appropriate selection of markers, for determining the reproducibility of the relative retention times (RRT), and for the accuracy of conversion factors (F). Finally, ten components were determined, with ganoderic acid A serving as single standard. Stable system parameters were established, and with successful resolution of those issues, this analytical method could be used more broadly.
超高效液相色谱法（UPLC）和多成分测定单标法（SSDMC）在草药质量控制中变得越来越重要；这种方法是针对灵芝开发的。在适当选择标志物、确定相对保留时间（RRT）的重现性以及转换因子（F）的准确性方面需要特别注意。最后，测定了十种成分，以灵芝酸A作为单标。建立了稳定的系统参数，并且成功解决了这些问题后，这种分析方法可以得到更广泛的应用。
