印度拉贾斯坦邦塔尔沙漠中（Forssk.）Exell的甲醇和乙酸乙酯根及茎提取物的气相色谱-质谱分析
Gas Chromatography Mass Spectrometry Profiling in Methanolic and Ethyl-acetate Root and Stem Extract of (Forssk.) Exell from Thar Desert of Rajasthan, India.
作者信息
Arora Sunita, Saini Manju
机构信息
Department of Botany, Jai Narain Vyas University, Jodhpur, Rajasthan, India.
出版信息
Pharmacognosy Res. 2017 Dec;9(Suppl 1):S48-S52. doi: 10.4103/pr.pr_62_17.
BACKGROUND
(Forssk.) Exell (), recently has moved to Lophiocarpaceae as per angiospermic plant group (APG) III system, is an annual or short-lived, dwarf, glabrous subshrub, prefers to grow on rocky places and on sand-stones in dry, hot areas of Rajasthan. This is the potential plant with medicinal properties. Vegetative organs under study show antioxidant, anti-inflammatory, antiulcer, antimicrobial, and antinociception activity.
OBJECTIVE
This study was carried out to identify the phytoconstituents present in the methanolic and ethyl-acetate extract of root and stem of by GC-MS analysis.
MATERIALS AND METHODS
Powdered test samples were sequentially extracted with methanol and ethyl-acetate. The compounds obtained as a result of GC-MS screening were identified on the basis of their retention time, peak area and compared with that of literature available and by interpretation of mass spectra.
RESULTS
GC-MS analysis of a methanolic extract of root detected mome-inositol (49.53%), guanosine (20.91%), and cis-vaccenic acid (9.25%). While ethyl-acetate extract of root analyzed pentadecanoic acid (17.91%), octadecanoic acid (15.01%) and cis-vaccenic acid (12.04%). Methanolic extract of stem detected mome-inositol (75.47%), pentadecanoic acid (6.04%), and 7-tetradecenal, (Z) (4.54%) while ethyl-acetate extract of stem revealed the presence of 1-heptacosanol (17.35%), hexadecanoic acid (17.17%), and octadecanal (12.64%).
CONCLUSION
The results suggest that (Forssk.) Exell is a plant of potential medicinal value, yielding various bioactive compounds that confirm the application of this plant as a plant-based drug in pharmacy-industry.
SUMMARY
Extraction is the most important step in the analysis of bioactive compounds present in botanical preparations. The strength of solvent plays a key role in this process, methanol as well as ethyl-acetate showed better response as far as extraction potency is concerned. Gas chromatography mass spectrometry analysis is highly reliable, and the interpretations of the results are of high-quality. This tool is in particular useful for confirming of the presence of bioactive-substances. The results suggest that (Forssk.) Exell can be used for drug formulations against some major disorders, i.e., cancer, ulcer, tuberculosis, arthritis, etc. GC-MS: Gas Chromatography-Mass Spectrometry, kPa: Kilopascal, RT: Retention time, MF: Molecular formula, MW: Molecular weight.
背景
根据被子植物种系发生学组（APG）III系统，（Forssk.）Exell最近已被归入角果藻科，它是一种一年生或寿命较短的矮小、无毛亚灌木，喜生长在拉贾斯坦邦干燥炎热地区的岩石地带和砂岩上。这是一种具有药用特性的潜在植物。所研究的营养器官显示出抗氧化、抗炎、抗溃疡、抗菌和抗伤害感受活性。
目的
本研究旨在通过气相色谱 - 质谱联用（GC - MS）分析确定（Forssk.）Exell根和茎的甲醇提取物及乙酸乙酯提取物中的植物成分。
材料与方法
将粉末状测试样品依次用甲醇和乙酸乙酯萃取。根据气相色谱 - 质谱联用筛选得到的化合物，依据其保留时间、峰面积进行鉴定，并与现有文献数据以及通过质谱解析结果进行比较。
结果
根的甲醇提取物的GC - MS分析检测到肌醇（49.53%）、鸟苷（20.91%）和顺式 - vaccenic酸（9.25%）。而根的乙酸乙酯提取物分析出十五烷酸（17.91%）、十八烷酸（15.01%）和顺式 - vaccenic酸（12.04%）。茎的甲醇提取物检测到肌醇（75.47%）、十五烷酸（6.04%）和（Z） - 7 - 十四碳烯醛（4.54%），而茎的乙酸乙酯提取物显示存在1 - 二十七醇（17.35%）、十六烷酸（17.17%）和十八醛（12.64%）。
结论
结果表明（Forssk.）Exell是一种具有潜在药用价值的植物，能产生多种生物活性化合物，证实了该植物在制药工业中作为植物源药物的应用。
总结
提取是植物制剂中生物活性化合物分析的最重要步骤。溶剂的强度在这个过程中起着关键作用，就提取效力而言，甲醇以及乙酸乙酯表现出更好的效果。气相色谱 - 质谱联用分析高度可靠，结果解读质量高。该工具对于确认生物活性物质的存在特别有用。结果表明（Forssk.）Exell可用于针对某些主要疾病（如癌症、溃疡、结核病、关节炎等）的药物制剂。GC - MS：气相色谱 - 质谱联用，kPa：千帕，RT：保留时间，MF：分子式，MW：分子量。
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