Department of Ultrasonography, First Hospital, Jilin University, Changchun, China.
Department of Neonatology, First Hospital, Jilin University, Changchun, China.
PLoS One. 2019 Nov 21;14(11):e0225463. doi: 10.1371/journal.pone.0225463. eCollection 2019.
The antiphospholipid syndrome (APS) is an important cause of acquired thromboembolic complications and pregnancy morbidity. The pathogenic mechanisms that damage the fetal-maternal unit and cause abnormal placental development are incompletely understood in APS patients. Liquid Chromatography/Mass Spectrometry (LC/MS) based metabolomics are applied for the mechanism of disease and further supporting the research of diagnosis and management in recent years. The aim of this research was to investigate the difference of serum metabolic profiles in recurrent abortion women with APS and healthy women to explore the mechanism of this disease.
Serum samples of 25 recurrent abortion women with APS and 25 healthy women were collected and analyzed by LC/MS in this study. Potential biomarkers were discovered by multivariate statistical analysis and then identified based on analysis results.
Totally, we identified five biomarkers that involved in different metabolic pathway such as purine metabolism, amino acid metabolism and tyrosine metabolism. These biomarkers showed different roles in disease development.
Metabolomics was proved as a powerful tool in understanding the mechanism of recurrent abortion caused by APS.
抗磷脂综合征（APS）是获得性血栓栓塞并发症和妊娠发病率的重要原因。APS 患者中，破坏胎儿-母体单位并导致胎盘发育异常的致病机制尚未完全阐明。近年来，基于液相色谱/质谱（LC/MS）的代谢组学被应用于疾病机制的研究，并为诊断和治疗提供了进一步的支持。本研究旨在探讨复发性流产合并 APS 患者与健康妇女血清代谢谱的差异，以探讨该疾病的发病机制。
本研究收集了 25 例复发性流产合并 APS 患者和 25 例健康妇女的血清样本，并通过 LC/MS 进行分析。通过多变量统计分析发现潜在的生物标志物，然后根据分析结果进行鉴定。
总共鉴定出 5 种生物标志物，它们涉及嘌呤代谢、氨基酸代谢和酪氨酸代谢等不同的代谢途径。这些生物标志物在疾病发展中表现出不同的作用。
代谢组学被证明是理解 APS 引起的复发性流产发病机制的有力工具。
