Post-Graduate Program, University of Fortaleza (Unifor), Brazil.
Post-Graduate Program, University of Fortaleza (Unifor) and Federal University of Ceará (UFC), Brazil.
Clin Appl Thromb Hemost. 2020 Jan-Dec;26:1076029620905338. doi: 10.1177/1076029620905338.
The aim of this study was to determine the plasma protein profile of patients with primary antiphospholipid syndrome (PAPS) compared to healthy controls and identify proteins that might be used in the evaluation, diagnosis, and prognosis of this condition. The sample consisted of 14 patients with PAPS and 17 sex- and age-matched controls. Plasma samples were submitted to proteomic analysis (albumin and immunoglobulin G depletion, concentration, digestion, and label-free data-independent mass spectrometry). The software Expression was used to quantify intergroup differences in protein expression. The analysis yielded 65 plasma proteins of which 11 were differentially expressed (9 upregulated and 2 downregulated) in relation to controls. Four of these are known to play a role in pathophysiological mechanisms of thrombosis: fibrinogen α chain, fibrinogen α chain, apolipoprotein C-III, and α-1-glycoprotein-1. Our analysis revealed autoimmune response and the presence of proteins believed to be functionally involved in the induction of procoagulant activity in patients with PAPS. Further studies are necessary to confirm our findings and may eventually lead to the development of significantly more accurate diagnostic tools.
本研究旨在确定原发性抗磷脂综合征（PAPS）患者与健康对照者的血浆蛋白谱，并鉴定可能用于评估、诊断和预测该疾病的蛋白。该样本包括 14 例 PAPS 患者和 17 名性别和年龄匹配的对照者。对血浆样本进行蛋白质组学分析（白蛋白和免疫球蛋白 G 耗尽、浓缩、消化和无标记数据独立质谱）。使用 Expression 软件对蛋白表达的组间差异进行定量。分析得到 65 种血浆蛋白，其中 11 种与对照组相比存在差异表达（9 种上调，2 种下调）。其中 4 种已知在血栓形成的病理生理机制中发挥作用：纤维蛋白原α链、纤维蛋白原α链、载脂蛋白 C-III 和α-1-糖蛋白-1。我们的分析揭示了自身免疫反应和存在被认为在诱导 PAPS 患者促凝活性方面具有功能的蛋白。需要进一步的研究来证实我们的发现，这可能最终导致开发出更准确的诊断工具。
