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利用对壮观霉素抗性的可选择标记在大肠杆菌中进行等位基因替换：lexA基因的替换

Allele replacement in Escherichia coli by use of a selectable marker for resistance to spectinomycin: replacement of the lexA gene.

作者信息

Hill S A, Little J W

机构信息

Department of Biochemistry and Molecular Biology, University of Arizona,Tucson 85721.

出版信息

J Bacteriol. 1988 Dec;170(12):5913-5. doi: 10.1128/jb.170.12.5913-5915.1988.

DOI:10.1128/jb.170.12.5913-5915.1988
PMID:2848016
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211701/
Abstract

We replaced the Escherichia coli lexA gene by a segment of DNA coding for resistance to spectinomycin and streptomycin. The use of this segment expands the range of selectable markers usable for allele replacement. The availability of this null lexA mutation will facilitate genetic analysis of lexA and the SOS regulon.

摘要

我们用一段编码对壮观霉素和链霉素抗性的DNA片段替换了大肠杆菌的lexA基因。该片段的使用扩展了可用于等位基因替换的选择标记范围。这种lexA无效突变的存在将有助于对lexA和SOS调控子进行遗传分析。

相似文献

1
Allele replacement in Escherichia coli by use of a selectable marker for resistance to spectinomycin: replacement of the lexA gene.利用对壮观霉素抗性的可选择标记在大肠杆菌中进行等位基因替换：lexA基因的替换
J Bacteriol. 1988 Dec;170(12):5913-5. doi: 10.1128/jb.170.12.5913-5915.1988.
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本文引用的文献

1
Sensitivity and Resistance to Spectinomycin in Escherichia coli.大肠杆菌对壮观霉素的敏感性和耐药性
J Bacteriol. 1969 Nov;100(2):939-47. doi: 10.1128/jb.100.2.939-947.1969.
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The SOS regulatory system of Escherichia coli.大肠杆菌的SOS调控系统。
Cell. 1982 May;29(1):11-22. doi: 10.1016/0092-8674(82)90085-x.
3
Nucleotide sequence of the lexA gene of E. coli.大肠杆菌lexA基因的核苷酸序列。
Cell. 1981 Mar;23(3):689-97. doi: 10.1016/0092-8674(81)90432-3.
4
Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.大肠杆菌中的诱变作用及对脱氧核糖核酸损伤的诱导反应
Microbiol Rev. 1984 Mar;48(1):60-93. doi: 10.1128/mr.48.1.60-93.1984.
5
Novel mechanism of cell division inhibition associated with the SOS response in Escherichia coli.大肠杆菌中与SOS反应相关的细胞分裂抑制新机制。
J Bacteriol. 1983 Oct;156(1):243-50. doi: 10.1128/jb.156.1.243-250.1983.
6
Escherichia coli RecBC pseudorevertants lacking chi recombinational hotspot activity.缺乏契合成重组热点活性的大肠杆菌RecBC假回复突变体。
J Bacteriol. 1983 Aug;155(2):664-80. doi: 10.1128/jb.155.2.664-680.1983.
7
Transcription analysis of the lexA gene of Escherichia coli: attenuation and cotranscription with the neighboring region.大肠杆菌lexA基因的转录分析：衰减作用及与相邻区域的共转录
Nucleic Acids Res. 1984 Jan 25;12(2):1203-17.
8
New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
9
The DNA sequences encoding plsB and dgk loci of Escherichia coli.
J Biol Chem. 1983 Sep 25;258(18):10856-61.
10
Isolation and characterization of Tn5 insertion mutations in the lexA gene of Escherichia coli.大肠杆菌lexA基因中Tn5插入突变的分离与鉴定
J Bacteriol. 1983 Mar;153(3):1368-78. doi: 10.1128/jb.153.3.1368-1378.1983.