Hill S A, Little J W
Department of Biochemistry and Molecular Biology, University of Arizona,Tucson 85721.
J Bacteriol. 1988 Dec;170(12):5913-5. doi: 10.1128/jb.170.12.5913-5915.1988.
We replaced the Escherichia coli lexA gene by a segment of DNA coding for resistance to spectinomycin and streptomycin. The use of this segment expands the range of selectable markers usable for allele replacement. The availability of this null lexA mutation will facilitate genetic analysis of lexA and the SOS regulon.
我们用一段编码对壮观霉素和链霉素抗性的DNA片段替换了大肠杆菌的lexA基因。该片段的使用扩展了可用于等位基因替换的选择标记范围。这种lexA无效突变的存在将有助于对lexA和SOS调控子进行遗传分析。