Anderson P
Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts 02115.
J Bacteriol. 1969 Nov;100(2):939-47. doi: 10.1128/jb.100.2.939-947.1969.
Inhibition of growth and division of Escherichia coli by spectinomycin is reversible, and the kinetics of its interference with deoxyribonucleic and ribonucleic acid synthesis may be interpreted as secondary effects of inhibition of protein synthesis on the ribosome. Spontaneous mutations to spectinomycin resistance occur in E. coli K-12 at a rate of about 2 x 10(-10). Resistance is transducible with a discrete lag in phenotypic expression, and the kinetics of its development is about the same as that for streptomycin resistance. All spectinomycin-resistant mutants tested contain resistant ribosomes, and all map in a locus (spc) counterclockwise to and 70% cotransducible with the classical str locus. Differences in the residual drug sensitivity of various spectinomycin-resistant mutants, and of their ribosomes, indicate the existence of more than one phenotypic class of resistance.
壮观霉素对大肠杆菌生长和分裂的抑制作用是可逆的，其干扰脱氧核糖核酸和核糖核酸合成的动力学可解释为对核糖体蛋白质合成抑制的次级效应。大肠杆菌K-12中对壮观霉素抗性的自发突变发生率约为2×10⁻¹⁰。抗性可通过转导传递，表型表达存在离散延迟，其发展动力学与链霉素抗性的动力学大致相同。所有测试的壮观霉素抗性突变体都含有抗性核糖体，且都定位在一个位点（spc），该位点与经典的str位点逆时针方向排列，且70%可共转导。各种壮观霉素抗性突变体及其核糖体的残留药物敏感性差异表明存在不止一种表型抗性类别。
