内转录间隔区（ITS），一种用于鉴别蔓龙胆属（龙胆科）植物物种的理想DNA条形码。
INTERNAL TRANSCRIBED SPACER (ITS), AN IDEAL DNA BARCODE FOR SPECIES DISCRIMINATION IN CRAWFURDIA WALL. (GENTIANACEAE).
作者信息
Zhang Dequan, Jiang Bei, Duan Lizhen, Zhou Nong
机构信息
College of pharmacy and chemistry, Dali University, Dali, Yunnan 671000, PR China.
Institute of Materia Medica, Dali University, Dali, Yunnan 671000, PR China.
出版信息
Afr J Tradit Complement Altern Med. 2016 Sep 29;13(6):101-106. doi: 10.21010/ajtcam.v13i6.15. eCollection 2016.
BACKGROUND
DNA barcoding is a technique used to identify species based on species-specific differences in short regions of their DNA. It is widely used in species discrimination of medicinal plants and traditional medicines.
MATERIALS AND METHODS
In the present study, four potential DNA barcodes, namely , , and ITS (nuclear ribosomal internal transcribed spacer) were adopted for species discrimination in Wall (Genetiaceae). Identification ability of these DNA barcodes and combinations were evaluated using three classic methods (Distance, Blast and Tree-Building).
RESULTS
As a result, ITS, and regions showed great universality for a success rate of 100%; whereas matK was disappointing for which only 65% samples gained useful DNA sequences. ITS region, which could clearly and effectively identify the five species in , performed very well in this study. On the contrary, and performed poorly in discrimination among these species.
CONCLUSION
ITS marker was an ideal DNA barcode in and it should be incorporated into one of the core barcodes for seed plants.
背景
DNA条形码是一种基于物种DNA短区域的物种特异性差异来识别物种的技术。它广泛应用于药用植物和传统药物的物种鉴别。
材料与方法
在本研究中，采用了四个潜在的DNA条形码，即 、 、 和ITS（核糖体DNA内转录间隔区）对 科的 进行物种鉴别。使用三种经典方法（距离法、Blast法和建树法）评估这些DNA条形码及其组合的识别能力。
结果
结果表明，ITS、 和 区域具有很高的通用性，成功率达100%；而matK则令人失望，只有65%的样本获得了有用的DNA序列。ITS区域能够清晰有效地鉴别出 中的五个物种，在本研究中表现出色。相反， 和 在这些物种的鉴别中表现不佳。
结论
ITS标记是 中理想的DNA条形码，应纳入种子植物核心条形码之一。
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