Ashrafi Behnam, Ramak Parvin, Ezatpour Behrouz, Talei Gholam Reza
Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran.
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences.
Afr J Tradit Complement Altern Med. 2017 Mar 1;14(3):209-217. doi: 10.21010/ajtcam.v14i3.23. eCollection 2017.
Boiss is a herb with wide-spread applications. Lorestan traditional healers have applied it for the treatment of rheumatoid diseases and stomach disorders.
Hydrodistillation process was used for essential oil extraction, the extracted essential oil was then analyzed through combination of capillary GC-FID, GC-MS and RI. The antimicrobial, antioxidant and cytotoxic activities of this essential oil were examined. Results indicate that the essential oil has a broad range of anti-microbial activity against all of the tested microorganisms.
The 50% of cytotoxic concentrations was 26.4 μg/ml and 4266.7 μg/ml for Hela cells and human lymphocytes, respectively. The oil cytotoxicity against the human tumor cell line was far higher than the amount required for human healthy cells. Conversely, the essential oil's IC value of 49.2 μg/ml in the DPPH assay, could be regarded as its strong antioxidant potential.
According to the data obtained, it can be concluded that essential oil could be applied as a safe antibacterial and antioxidant agent for food and pharmaceutical purposes.
博伊斯是一种应用广泛的草药。洛雷斯坦传统治疗师已将其用于治疗类风湿疾病和胃部疾病。
采用水蒸馏法提取精油，然后通过毛细管气相色谱 - 氢火焰离子化检测器（GC - FID）、气相色谱 - 质谱联用仪（GC - MS）和保留指数（RI）相结合的方法对提取的精油进行分析。检测了该精油的抗菌、抗氧化和细胞毒性活性。结果表明，该精油对所有测试微生物均具有广泛的抗菌活性。
对海拉细胞和人淋巴细胞的细胞毒性半数浓度分别为26.4μg/ml和4266.7μg/ml。该精油对人肿瘤细胞系的细胞毒性远高于对人健康细胞所需的量。相反，该精油在二苯基苦味酰基自由基（DPPH）测定中的半数抑制浓度（IC）值为49.2μg/ml，可被视为其强大的抗氧化潜力。
根据所获得的数据，可以得出结论，该精油可作为食品和制药用途的安全抗菌和抗氧化剂。
