Mohammed J R, Mohammed B S, Pawluk L J, Bucci D M, Baker N R, Davis W B
Department of Internal Medicine, Ohio State University, Columbus 43210.
J Lab Clin Med. 1988 Dec;112(6):711-20.
The neutrophil myeloperoxidase-H2O2-halide enzyme system produces hypochlorous acid and chlorinated amine compounds capable of killing a variety of target cells. In the present study we hypothesized that the myeloperoxidase enzyme system is one mechanism for airway epithelial damage in patients with cystic fibrosis (CF). Enzyme linked immunosorbent assay detected high antigenic levels of myeloperoxidase in sputum samples of seven patients with CF. Myeloperoxidase was purified to homogeneity from CF sputum and from blood neutrophils by a three-step technique involving dialysis, gel filtration, and ion-exchange chromatography. CF sputum myeloperoxidase and neutrophil myeloperoxidase appeared identical by acid gel electrophoresis and Ouchterlony experiments. CF sputum myeloperoxidase also contained approximately the same enzymatic activity as neutrophil myeloperoxidase. The myeloperoxidase enzyme system was tested for its cytotoxic potential in a tracheal ring culture system. Myeloperoxidase-induced cytotoxicity for airway epithelium was confirmed by light microscopy and radiolabelling experiments. These findings suggest a possible role for neutrophil myeloperoxidase in CF lung disease.
中性粒细胞髓过氧化物酶-H₂O₂-卤化物酶系统可产生次氯酸和氯化胺化合物，这些化合物能够杀死多种靶细胞。在本研究中，我们假设髓过氧化物酶系统是囊性纤维化（CF）患者气道上皮损伤的一种机制。酶联免疫吸附测定法检测到7例CF患者痰液样本中髓过氧化物酶的抗原水平较高。通过涉及透析、凝胶过滤和离子交换色谱的三步技术，从CF痰液和血液中性粒细胞中纯化髓过氧化物酶至均一性。通过酸性凝胶电泳和双向免疫扩散实验，CF痰液髓过氧化物酶和中性粒细胞髓过氧化物酶看起来是相同的。CF痰液髓过氧化物酶也含有与中性粒细胞髓过氧化物酶大致相同的酶活性。在气管环培养系统中测试了髓过氧化物酶系统的细胞毒性潜力。通过光学显微镜和放射性标记实验证实了髓过氧化物酶对气道上皮的细胞毒性作用。这些发现提示中性粒细胞髓过氧化物酶在CF肺部疾病中可能发挥作用。
