Wang Yaping, Qian Junmin, Liu Ting, Xu Weijun, Zhao Na, Suo Aili
State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
Mater Sci Eng C Mater Biol Appl. 2017 Jul 1;76:313-318. doi: 10.1016/j.msec.2017.03.098. Epub 2017 Mar 14.
Though much progress in utilizing tissue engineering technology to investigate tumor development in vitro has been made, the effective management of human melanoma is still a challenge in clinic due to lack of suitable 3D culture systems. In this study, we prepared a poly(γ-benzyl-l-glutamate)/poly(lactic acid) (PBLG/PLA) nanofiber membrane by electrospinning and demonstrated its suitability as a matrix for 3D culture of melanoma cells in vitro. The electrospun PBLG/PLA nanofiber membrane displayed a smooth and uniform fibrous morphology and had a desirable water contact angle of 79.3±0.6°. The average diameter of PBLG/PLA nanofibers was 320.3±95.1nm that was less than that (516.2±163.3nm) of pure PLA nanofibers. The addition of PBLG into PLA decreased the cold crystallization peak of PLA fibers from 93 to 75°C. The in vitro biocompatibility of PBLG/PLA nanofiber membrane was evaluated with B16F10 cells using PLA nanofiber membrane as control. It was found that, compared to PLA nanofiber membrane, PBLG/PLA nanofiber membrane could better support cell viability and proliferation, as indicated by MTT assay and live-dead staining. SEM results revealed that PBLG/PLA rather than PLA nanofiber membrane promoted the generation of tumoroid-like structures. These findings clearly demonstrated that the electrospun PBLG/PLA nanofiber membrane could mimick the extracellular matrix of melanoma microenvironment and be a promising platform for 3D cell culture.
尽管在利用组织工程技术体外研究肿瘤发展方面已取得很大进展，但由于缺乏合适的三维培养系统，人类黑色素瘤的有效管理在临床上仍是一项挑战。在本研究中，我们通过静电纺丝制备了聚（γ-苄基-L-谷氨酸）/聚乳酸（PBLG/PLA）纳米纤维膜，并证明其适合作为黑色素瘤细胞体外三维培养的基质。静电纺丝的PBLG/PLA纳米纤维膜呈现出光滑且均匀的纤维形态，具有79.3±0.6°的理想水接触角。PBLG/PLA纳米纤维的平均直径为320.3±95.1nm，小于纯PLA纳米纤维的直径（516.2±163.3nm）。向PLA中添加PBLG使PLA纤维的冷结晶峰从93℃降至75℃。以PLA纳米纤维膜为对照，用B16F10细胞评估PBLG/PLA纳米纤维膜的体外生物相容性。发现与PLA纳米纤维膜相比，PBLG/PLA纳米纤维膜能更好地支持细胞活力和增殖，MTT法和活死染色表明了这一点。扫描电子显微镜结果显示，PBLG/PLA而非PLA纳米纤维膜促进了类肿瘤样结构的生成。这些发现清楚地表明，静电纺丝的PBLG/PLA纳米纤维膜可模拟黑色素瘤微环境的细胞外基质，是一个有前景的三维细胞培养平台。
