利用红薯简便合成用于铁传感和细胞成像的荧光碳点。
Facile synthesis of fluorescence carbon dots from sweet potato for Fe sensing and cell imaging.
作者信息
Shen Jie, Shang Shaoming, Chen Xiuying, Wang Dan, Cai Yan
机构信息
School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China.
School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China.
出版信息
Mater Sci Eng C Mater Biol Appl. 2017 Jul 1;76:856-864. doi: 10.1016/j.msec.2017.03.178. Epub 2017 Mar 22.
In this study, a facile synthesis of fluorescence carbon dots (CDs) from sweet potato was performed through hydrothermal treatment. The obtained CDs with quantum yield of 8.64% have good dispersibility due to the soluble functional groups on their surfaces. The characterization of CDs was carried out and their possible formation mechanism was also discussed. In addition, the cytotoxicity results showed that the CDs exhibit non toxicity within 100μg/mL. At this concentration, the CDs were applied in cell imaging, indicating that they are promising fluorescent probes for biological imaging. In addition, the fluorescence of CDs was quenched by Fe with a linear concentration of 1 to 100μM, associated with the limit of detection of 0.32μM. Subsequently, the CDs were successfully applied for Fe probing in living cells.
在本研究中，通过水热处理从红薯中简便地合成了荧光碳点（CDs）。所获得的量子产率为8.64%的CDs由于其表面的可溶性官能团而具有良好的分散性。对CDs进行了表征，并讨论了其可能的形成机制。此外，细胞毒性结果表明，CDs在100μg/mL以内表现出无毒性。在此浓度下，CDs被应用于细胞成像，表明它们是用于生物成像的有前景的荧光探针。此外，CDs的荧光被Fe猝灭，线性浓度范围为1至100μM，检测限为0.32μM。随后，CDs成功地应用于活细胞中的Fe探测。
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