Sakura Takeshi, Morioka Tomoaki, Shioi Atsushi, Kakutani Yoshinori, Miki Yuya, Yamazaki Yuko, Motoyama Koka, Mori Katsuhito, Fukumoto Shinya, Shoji Tetsuo, Emoto Masanori, Inaba Masaaki
Department of Metabolism, Endocrinology and Molecular Medicine, Osaka City University Graduate School of Medicine, 1-4-3, Asahi-machi, Abeno-ku, Osaka, 545-8585, Japan.
Department of Vascular Medicine, Osaka City University Graduate School of Medicine, 1-4-3, Asahi-machi, Abeno-ku, Osaka, 545-8585, Japan.
Cardiovasc Diabetol. 2017 May 10;16(1):62. doi: 10.1186/s12933-017-0545-3.
Lipopolysaccharide (LPS)-binding protein (LBP) is an acute-phase reactant that mediates immune responses triggered by LPS. Recent evidence indicates the association of circulating LBP levels with obesity, diabetes, and cardiovascular diseases. In this study, we aimed to investigate the relationship between serum LBP levels and arterial stiffness in patients with type 2 diabetes.
A total of 196 patients with type 2 diabetes, including 101 men and 95 women, were enrolled in this cross-sectional study. Fasting serum LBP levels were determined by enzyme-linked immunosorbent assay. Arterial stiffness was assessed by measuring the aortic pulse wave velocity (PWV).
The mean values of serum LBP and aortic PWV were 18.2 μg/mL and 1194 cm/s, respectively. Serum LBP levels were positively correlated with body mass index, triglycerides, high-sensitivity C-reactive protein, and insulin resistance index and were negatively correlated with high-density lipoprotein cholesterol. They were, however, not significantly correlated with aortic PWV in univariate analyses. Multivariate analysis revealed that serum LBP levels were independently and positively associated with aortic PWV (β = 0.135, p = 0.026) after adjusting for age, sex, body mass index, albumin, high-sensitivity C-reactive protein, and other cardiovascular risk factors. Further analyses revealed that the impact of serum LBP levels on aortic PWV was modified by sex, and the association between serum LBP levels and aortic PWV was found to be significant only in men.
Serum LBP levels are associated with arterial stiffness, independent of obesity and traditional cardiovascular risk factors, especially in men with type 2 diabetes. This study indicates a potential role of the LPS/LBP-induced innate immunity in the development and progression of arterial stiffness in type 2 diabetes.
脂多糖（LPS）结合蛋白（LBP）是一种急性期反应物，可介导由LPS触发的免疫反应。最近的证据表明循环LBP水平与肥胖、糖尿病和心血管疾病有关。在本研究中，我们旨在调查2型糖尿病患者血清LBP水平与动脉僵硬度之间的关系。
本横断面研究共纳入196例2型糖尿病患者，其中男性101例，女性95例。采用酶联免疫吸附测定法测定空腹血清LBP水平。通过测量主动脉脉搏波速度（PWV）评估动脉僵硬度。
血清LBP和主动脉PWV的平均值分别为18.2μg/mL和1194cm/s。血清LBP水平与体重指数、甘油三酯、高敏C反应蛋白和胰岛素抵抗指数呈正相关，与高密度脂蛋白胆固醇呈负相关。然而，在单变量分析中，它们与主动脉PWV无显著相关性。多变量分析显示，在调整年龄、性别、体重指数、白蛋白、高敏C反应蛋白和其他心血管危险因素后，血清LBP水平与主动脉PWV独立正相关（β=0.135，p=0.026）。进一步分析显示，血清LBP水平对主动脉PWV的影响存在性别差异，血清LBP水平与主动脉PWV之间的关联仅在男性中显著。
血清LBP水平与动脉僵硬度有关，独立于肥胖和传统心血管危险因素，尤其是在2型糖尿病男性中。本研究表明LPS/LBP诱导的固有免疫在2型糖尿病动脉僵硬度的发生和发展中具有潜在作用。
