培养基成分对人源肝脏Hep G2细胞系药物代谢酶活性的影响。
The influence of culture medium composition on drug metabolising enzyme activities of the human liver derived Hep G2 cell line.
作者信息
Doostdar H, Duthie S J, Burke M D, Melvin W T, Grant M H
机构信息
Department of Medicine & Therapeutics, University of Aberdeen, Scotland.
出版信息
FEBS Lett. 1988 Dec 5;241(1-2):15-8. doi: 10.1016/0014-5793(88)81021-4.
When grown in the standard Dulbecco's medium the human liver derived Hep G2 hepatoma cell line shows only 10-20% of the cytochrome P-450-dependent mixed function oxidase (MFO) activity of freshly isolated human adult hepatocytes. However, the MFO activities and, to a lesser extent, the activities of UDP-glucuronyltransferase and glutathione-S-transferase can be increased by altering the composition of the growth medium. Modified Earle's medium was more effective in this respect than Williams' E medium and increased the O-dealkylations of ethoxyresorufin, benzyloxyresorufin and pentoxyresorufin 50-, 30- and 10-fold, respectively.
在标准的杜尔贝科培养基中培养时，源自人肝脏的Hep G2肝癌细胞系的细胞色素P - 450依赖性混合功能氧化酶（MFO）活性仅为新鲜分离的成人肝细胞的10% - 20%。然而，通过改变生长培养基的成分，可以提高MFO活性，在较小程度上也能提高尿苷二磷酸葡萄糖醛酸基转移酶和谷胱甘肽 - S - 转移酶的活性。在这方面，改良的厄尔培养基比威廉姆斯E培养基更有效，分别使乙氧试卤灵、苄氧试卤灵和戊氧试卤灵的O - 脱烷基化增加了50倍、30倍和10倍。
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