Yunnan Provincial Key Laboratory of Panax notoginseng, Key Laboratory of Panax notoginseng Resources Sustainable Development and Utilization of State Administration of Traditional Chinese Medicine, Kunming Key Laboratory of Sustainable Development and Utilization of Famous-Region Drug, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
Centre for Microelement Research of Huazhong Agricultural University, Wuhan, 430070, China.
Sci Rep. 2017 May 9;7(1):1620. doi: 10.1038/s41598-017-01803-3.
An isobaric tags for relative and absolute quantitative (iTRAQ)-based quantitative proteomic approach was used to screen the differentially expressed proteins during control treatment (CK), aluminum (Al) and Al indole-3-acetic acid (IAA) treatment of wheat lines ET8 (Al-tolerant). Further, the the expression levels of auxin response factor (ARF), Aux/IAA, Mitogen activated protein kinase (MAPK) 2c, and MAPK1a were analyzed. Results showed that 16 proteins were determined to be differentially expressed in response to Al and IAA co-treatment compared with Al alone. Among them, MAPK2c and MAPK1a proteins displayed markedly differential expression during the processes. The expression of ARF2 was upregulated and Aux/IAA was downregulated by Al, while both in concentration- and time-dependent manners. Western-blot detection of MAPK2c and MAPK1a indicated that Al upregulated MAPK2c and downregulated MAPK1a in both concentration- and time-dependent manners. Exogenous IAA could promote the expression of MAPK2c, but inhibit the expression of MAPK1a in the presence/absence of Al. These findings indicated that IAA acted as one of the key signaling molecule controls the response mechanism of wheat malic acid efflux to Al stress through the suppression/activation of Aux/IAA and ARFs, and the activity of MAPK2c and MAPK1a were positively or negatively regulated.
采用等压标签相对和绝对定量（iTRAQ）技术的定量蛋白质组学方法筛选了小麦 ET8（耐铝）品系在对照处理（CK）、铝（Al）和铝吲哚-3-乙酸（IAA）处理下差异表达的蛋白质。进一步分析了生长素响应因子（ARF）、Aux/IAA、丝裂原活化蛋白激酶（MAPK）2c 和 MAPK1a 的表达水平。结果表明，与单独 Al 处理相比，16 种蛋白质在 Al 和 IAA 共同处理时被确定为差异表达。其中，MAPK2c 和 MAPK1a 蛋白在这一过程中表现出明显的差异表达。Al 处理上调了 ARF2 的表达，下调了 Aux/IAA 的表达，且呈浓度和时间依赖性。MAPK2c 和 MAPK1a 的 Western-blot 检测表明，Al 以浓度和时间依赖性方式上调 MAPK2c，下调 MAPK1a 的表达。外源性 IAA 可促进 MAPK2c 的表达，但在存在/不存在 Al 的情况下抑制 MAPK1a 的表达。这些发现表明，IAA 作为关键信号分子之一，通过抑制/激活 Aux/IAA 和 ARFs 来控制小麦苹果酸外排对 Al 胁迫的反应机制，MAPK2c 和 MAPK1a 的活性受到正向或负向调节。
