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不同种植体周围炎治疗后取出的牙科种植体的生物膜分析

Biofilm Analysis of Retrieved Dental Implants after Different Peri-Implantitis Treatments.

作者信息

Geremias Thaise C, Montero Juan F D, Magini Ricardo de Souza, Schuldt Filho Guenther, de Magalhães Edival Barreto, Bianchini Marco A

机构信息

School of Dentistry, Center for Research on Dental Implants, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

School of Dentistry, UniSul University, Florianópolis, SC, Brazil.

出版信息

Case Rep Dent. 2017;2017:8562050. doi: 10.1155/2017/8562050. Epub 2017 Apr 9.

Abstract

The aim of the current study was to analyse the planktonic growth of on the surfaces of three implants retrieved after three different peri-implantitis treatments. Three implants from a male patient with high levels of bone loss were treated by mechanical debridement, chemical decontamination, and implantoplasty. After 4 months of follow-up, the implants were removed. The growth and biofilm formation were measured by spectrophotometry (OD) and scanning electron microscopy (SEM), after 48 hours of incubation. Results showed an average of planktonic growth over the implants of 0.21 nm (mechanical debridement), 0.16 nm (chemical decontamination), and 0.15 nm (implantoplasty). Data were analysed by ANOVA and Tukey's test ( < 0.05 for chemical decontamination and implantoplasty). Implantoplasty and chemical decontamination showed the lowest levels of planktonic growth, indicating a possible influence of the modification procedures on the titanium surface on the initial biofilm attachment.

摘要

本研究的目的是分析在三种不同种植体周围炎治疗后取出的三种种植体表面上的浮游生物生长情况。对一名骨丢失严重的男性患者的三个种植体进行了机械清创、化学去污和种植体成形术治疗。随访4个月后,取出种植体。在孵育48小时后,通过分光光度法(OD)和扫描电子显微镜(SEM)测量生长和生物膜形成情况。结果显示,种植体上的浮游生物平均生长量分别为0.21nm(机械清创)、0.16nm(化学去污)和0.15nm(种植体成形术)。数据通过方差分析和Tukey检验进行分析(化学去污和种植体成形术的P<0.05)。种植体成形术和化学去污显示出最低水平的浮游生物生长,表明钛表面的改性程序对初始生物膜附着可能有影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837b/5401748/d60617b500b2/CRID2017-8562050.001.jpg

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