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草药原料的真菌学分析及黄曲霉毒素B污染物估计

MYCOLOGICAL ANALYSIS AND AFLATOXIN B CONTAMINANT ESTIMATION OF HERBAL DRUG RAW MATERIALS.

作者信息

Rajeshwari Puttaswamy, Raveesha KoteshwarAnandrao

机构信息

Centre for Innovative Studies in Herbal Drug Technology, Department of Studies in Botany, University of Mysore, Mysuru-570006 India.

出版信息

Afr J Tradit Complement Altern Med. 2016 Aug 12;13(5):123-131. doi: 10.21010/ajtcam.v13i5.16. eCollection 2016.

DOI:10.21010/ajtcam.v13i5.16
PMID:28487902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5416630/
Abstract

BACKGROUND

The present study explores the fungal contamination of important herbal drug raw materials (HDRM), which are widely used in the preparation of many herbal drugs. Understanding of the microbial contamination status of HDRM is one of the important steps to ensure the safety and efficacy of herbal drugs.

MATERIALS AND METHODS

Eighteen samples of six herbal drug raw materials (HDRM) viz., Linn., Vahl., (Linn.) Urban, Houtt., (Wild) Miers and (Linn.) Dunal, were screened for fungal contamination, by employing serial dilution method. All the isolates of were screened for their ability to produce aflatoxin B (AB) and highly contaminated samples were subjected to AB estimation by using Thin Layer Chromatography (TLC), spectrophotometric method and occurrence of Aflatoxin B was confirmed by Liquid Chromatography-Mass Spectrometry analysis (LCMS).

RESULTS

A total of 302 isolates of 42 fungal species belonging to 17 genera were found in association with test the samples. More than 61% of isolates tested positive for production of AB and highest yield recorded was 5008.20 ppb from the isolates of . Amongthesix highly contaminated samples three samples tested positive for AB. Highest AB was recorded from (104.19 μg/kg), followed by (13.73 μg/kg) and (12.02 μg/kg).

CONCLUSION

Assessment of fungal and mycotoxin contamination should be a part of the quality check while selecting HDRM for manufacture of herbal products. Safe processing and storage practices are necessary.

摘要

背景

本研究探讨了重要草药原料（HDRM）的真菌污染情况，这些原料广泛用于多种草药的制备。了解HDRM的微生物污染状况是确保草药安全性和有效性的重要步骤之一。

材料与方法

采用系列稀释法，对六种草药原料（HDRM）的18个样本进行真菌污染筛选，这六种原料分别为[此处六种原料名称未给出英文，无法准确翻译]。对所有分离出的[此处名称未给出英文，无法准确翻译]菌株进行黄曲霉毒素B（AB）产生能力的筛选，对高度污染的样本采用薄层色谱法（TLC）、分光光度法进行AB含量测定，并通过液相色谱 - 质谱分析（LCMS）确认黄曲霉毒素B的存在。

结果

在测试样本中共发现了属于17个属的42种真菌的302个分离株。超过61%的[此处名称未给出英文，无法准确翻译]分离株AB产生测试呈阳性，记录到的最高产量为来自[此处名称未给出英文，无法准确翻译]分离株的5008.20 ppb。在六个高度污染的样本中，有三个样本AB检测呈阳性。AB含量最高的是[此处名称未给出英文，无法准确翻译]（104.19 μg/kg），其次是[此处名称未给出英文，无法准确翻译]（13.73 μg/kg）和[此处名称未给出英文，无法准确翻译]（12.02 μg/kg）。

结论

在选择用于生产草药产品的HDRM时，真菌和霉菌毒素污染评估应作为质量检查的一部分。安全的加工和储存做法是必要的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94b6/5416630/b6ba25a9d1be/AJTCAM-13-123-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94b6/5416630/3212e18d7139/AJTCAM-13-123-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94b6/5416630/99f9e041b83b/AJTCAM-13-123-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94b6/5416630/ac2510e16681/AJTCAM-13-123-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94b6/5416630/6cc489e751fc/AJTCAM-13-123-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94b6/5416630/b6ba25a9d1be/AJTCAM-13-123-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94b6/5416630/3212e18d7139/AJTCAM-13-123-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94b6/5416630/99f9e041b83b/AJTCAM-13-123-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94b6/5416630/ac2510e16681/AJTCAM-13-123-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94b6/5416630/6cc489e751fc/AJTCAM-13-123-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94b6/5416630/b6ba25a9d1be/AJTCAM-13-123-g008.jpg

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