Catal Tunc, Gover Tansu, Yaman Bugra, Droguetti Jessica, Yilancioglu Kaan
Department of Molecular Biology and Genetics, Uskudar University, Uskudar, 34662, Istanbul, Turkey.
Biotechnology Application and Research Center, Uskudar University, Uskudar, 34662, Istanbul, Turkey.
World J Microbiol Biotechnol. 2017 Jun;33(6):115. doi: 10.1007/s11274-017-2270-1. Epub 2017 May 9.
Microbial electrochemical cells including microbial fuel cells (MFCs) and microbial electrolysis cells (MECs) are novel biotechnological tools that can convert organic substances in wastewater or biomass into electricity or hydrogen. Electroactive microbial biofilms used in this technology have ability to transfer electrons from organic compounds to anodes. Evaluation of biofilm formation on anode is crucial for enhancing our understanding of hydrogen generation in terms of substrate utilization by microorganisms. In this study, furfural and hydroxymethylfurfural (HMF) were analyzed for hydrogen generation using single chamber membrane-free MECs (17 mL), and anode biofilms were also examined. MECs were inoculated with mixed bacterial culture enriched using chloroethane sulphonate. Hydrogen was succesfully produced in the presence of HMF, but not furfural. MECs generated similar current densities (5.9 and 6 mA/cm furfural and HMF, respectively). Biofilm samples obtained on the 24th and 40th day of cultivation using aromatic compounds were evaluated by using epi-fluorescent microscope. Our results show a correlation between biofilm density and hydrogen generation in single chamber MECs.
包括微生物燃料电池（MFC）和微生物电解池（MEC）在内的微生物电化学电池是新型生物技术工具，可将废水中的有机物质或生物质转化为电能或氢气。该技术中使用的电活性微生物生物膜具有将电子从有机化合物转移到阳极的能力。评估阳极上生物膜的形成对于增强我们对微生物利用底物产生氢气的理解至关重要。在本研究中，使用单室无膜MEC（17毫升）分析了糠醛和羟甲基糠醛（HMF）的产氢情况，并对阳极生物膜进行了检测。MEC接种了用氯乙烷磺酸盐富集的混合细菌培养物。在HMF存在下成功产生了氢气，但糠醛存在时未产生氢气。MEC产生了相似的电流密度（糠醛和HMF分别为5.9和6 mA/cm）。使用落射荧光显微镜对在培养第24天和第40天使用芳香族化合物获得的生物膜样品进行了评估。我们的结果表明单室MEC中生物膜密度与产氢之间存在相关性。
