Kilinc Burak, Akagunduz Dilan, Ozdemir Murat, Kul Aykut, Catal Tunc
Istanbul Protein Research-Application and Inovation Center (PROMER), Uskudar University, 34662 Uskudar, Istanbul Turkey.
Personalized Medicine Application and Research Center (KIMER), Uskudar University, 34662 Uskudar, Istanbul Turkey.
3 Biotech. 2023 Nov;13(11):382. doi: 10.1007/s13205-023-03805-7. Epub 2023 Oct 31.
In this study, the effects of cocaine metabolite, benzoylecgonine, commonly found in wastewater on hydrogen production were investigated using microbial electrolysis cells. Benzoylecgonine dissolved in synthetic urine and human urine containing benzoylecgonine were inoculated to evaluate hydrogen production performance in microbial electrolysis cells. Microbial electrolysis cells were inoculated with synthetic urine and human urine containing the cocaine metabolite benzoylecgonine for hydrogen gas production performance. Gas production was observed and measured daily by gas chromatography. GC-MS was used to analyze the compounds found in human urine before and after operation in microbial electrolysis cells. The metabolite's pH values and optical density in microbial electrolysis cells were analyzed spectrophotometrically. Hydrogen gas was successfully produced in microbial electrolysis cells (~ 5.5 mL) at the end of the 24th day in the presence of benzoylecgonine in synthetic urine. Human urine containing benzoylecgonine also generated hydrogen in microbial electrolysis cells. In conclusion, microbial electrolysis cells can be used to remove cocaine metabolites from contaminated wastewater generating hydrogen gas.
The online version contains supplementary material available at 10.1007/s13205-023-03805-7.
在本研究中，使用微生物电解池研究了废水中常见的可卡因代谢物苯甲酰芽子碱对产氢的影响。将溶解在合成尿液和含有苯甲酰芽子碱的人尿液中的苯甲酰芽子碱接种，以评估微生物电解池中的产氢性能。用含有可卡因代谢物苯甲酰芽子碱的合成尿液和人尿液接种微生物电解池以进行产氢性能测试。每天通过气相色谱法观察和测量气体产生情况。使用气相色谱 - 质谱联用仪分析微生物电解池运行前后人尿液中发现的化合物。用分光光度法分析微生物电解池中代谢物的pH值和光密度。在合成尿液中存在苯甲酰芽子碱的情况下，在第24天结束时微生物电解池中成功产生了氢气（约5.5毫升）。含有苯甲酰芽子碱的人尿液在微生物电解池中也产生了氢气。总之，微生物电解池可用于从受污染的废水中去除可卡因代谢物并产生氢气。
在线版本包含可在10.1007/s13205-023-03805-7获取的补充材料。
