Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia.
University of New South Wales, Sydney, NSW 2052, Australia.
Nat Rev Mol Cell Biol. 2017 Jun;18(6):339-340. doi: 10.1038/nrm.2017.49. Epub 2017 May 10.
RNA modifications can alter RNA structure-function relationships and various cellular processes. However, the genomic distribution and biological roles of most RNA modifications remain uncharacterized. Here, we propose using phage display antibody technology and direct sequencing through nanopores to facilitate systematic interrogation of the distribution, location and dynamics of RNA modifications.
RNA 修饰可以改变 RNA 的结构-功能关系和各种细胞过程。然而，大多数 RNA 修饰的基因组分布和生物学作用仍未被描述。在这里，我们提出使用噬菌体展示抗体技术和通过纳米孔进行直接测序，以促进对 RNA 修饰的分布、位置和动态的系统研究。
