Khan Imran, Kang Sun Chul
Dept. of Biotechnology, Daegu Univ., Gyeongsan, Gyeongbuk, 38453, Republic of Korea.
J Food Sci. 2017 Jun;82(6):1475-1483. doi: 10.1111/1750-3841.13732. Epub 2017 May 10.
Fermented food has been always possesses upper hand compared to normal food due to its antibacterial, antioxidant, and anticancer properties. Soybeans, which have high nutritional value, are widely consumed in Korea. In this study, soybean seed powder fermented with Lactobacillus plantarum DGK-17, which was previously isolated from kimchi, showed anticancer potential. Fermented soybean extract (FSE) resulted in morphological changes, reduction of cancer cell colony formation and apoptotic cell death of HCT-116 colon cancer cells in a dose-dependent manner, and IC value of 111 μg. FSE treatment caused reduction of cell growth in a dose-dependent manner via release of lactate dehydrogenase. FSE treatment induced HCT-116 apoptotic cell death as confirmed by the presence of fragmented nuclei, oxidative burst, and reduced mitochondrial membrane potential (ΔΨ ). Further, FSE treatment sensitized cells to ER stress via IRE1-α induction. FSE treatment also resulted in JNK activation, subsequently causing activation of Bax and downregulation of BCl2. Weakened mitochondrial membrane potential (ΔΨ ) also caused release of Cyto C, further activating caspase-mediated cell death. Therefore, this study reveals the apoptotic role of DGK-17-fermented soybean seed extract in human colon cancer HCT-116 cells.
由于具有抗菌、抗氧化和抗癌特性，发酵食品一直比普通食品更具优势。大豆营养价值高，在韩国被广泛食用。在本研究中，用先前从泡菜中分离出的植物乳杆菌DGK - 17发酵的大豆种子粉显示出抗癌潜力。发酵大豆提取物（FSE）导致HCT - 116结肠癌细胞出现形态变化、癌细胞集落形成减少和凋亡性细胞死亡，且呈剂量依赖性，IC值为111μg。FSE处理通过释放乳酸脱氢酶以剂量依赖性方式导致细胞生长减少。FSE处理诱导HCT - 116凋亡性细胞死亡，细胞核碎片化、氧化爆发以及线粒体膜电位（ΔΨ）降低证实了这一点。此外，FSE处理通过诱导IRE1 - α使细胞对内质网应激敏感。FSE处理还导致JNK激活，随后引起Bax激活和BCl2下调。线粒体膜电位（ΔΨ）减弱也导致细胞色素C释放，进一步激活半胱天冬酶介导的细胞死亡。因此，本研究揭示了DGK - 17发酵大豆种子提取物在人结肠癌HCT - 116细胞中的凋亡作用。
