Wechsler S L, Nesburn A B, Watson R, Slanina S, Ghiasi H
Ophthalmology Research, Cedars-Sinai Medical Center, Los Angeles, California 90048.
J Gen Virol. 1988 Dec;69 ( Pt 12):3101-6. doi: 10.1099/0022-1317-69-12-3101.
In situ hybridization with synthetic oligonucleotides was used to fine map the extent and continuity of herpes simplex virus type 1 (HSV-1) latency-related RNA (LR RNA) in trigeminal ganglia of latently infected humans. A series of 34 20 residue oligonucleotides were employed to cover a 3 kb region of the HSV-1 genome to which the latency-related gene had previously been mapped. The 5' end of the main LR RNA transcript began approximately 1210 nucleotides downstream from the 3' end of the mRNA from the immediate early gene ICP0. The 3' end of the LR RNA overlapped the 3' end of the ICP0 mRNA by approximately 1000 nucleotides. A potential small intron was detected near the 3' end of the LR RNA.
采用合成寡核苷酸原位杂交技术，对潜伏感染人类三叉神经节中1型单纯疱疹病毒（HSV-1）潜伏相关RNA（LR RNA）的范围和连续性进行精细定位。使用一系列34个20个残基的寡核苷酸覆盖HSV-1基因组中先前已定位潜伏相关基因的3 kb区域。主要LR RNA转录本的5'端起始于紧邻早期基因ICP0的mRNA 3'端下游约1210个核苷酸处。LR RNA的3'端与ICP0 mRNA的3'端重叠约1000个核苷酸。在LR RNA的3'端附近检测到一个潜在的小内含子。
