Dong Yating, Liu Hui, Zhang Yi, Hu Jiahui, Feng Jiyu, Li Cong, Li Cheng, Chen Jinhong, Zhu Shuijin
Department of Agronomy, Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, China.
PLoS One. 2017 May 10;12(5):e0176733. doi: 10.1371/journal.pone.0176733. eCollection 2017.
Aldehyde dehydrogenases (ALDHs) are a superfamily of enzymes which play important role in the scavenging of active aldehydes molecules. In present work, a comprehensive whole-genomic study of ALDH gene superfamily was carried out for an allotetraploid cultivated cotton species, G. hirsutum, as well as in parallel relative to their diploid progenitors, G. arboreum and G. raimondii. Totally, 30 and 58 ALDH gene sequences belong to 10 families were identified from diploid and allotetraploid cotton species, respectively. The gene structures among the members from same families were highly conserved. Whole-genome duplication and segmental duplication might be the major driver for the expansion of ALDH gene superfamily in G. hirsutum. In addition, the expression patterns of GhALDH genes were diverse across tissues. Most GhALDH genes were induced or repressed by salt stress in upland cotton. Our observation shed lights on the molecular evolutionary properties of ALDH genes in diploid cottons and their alloallotetraploid derivatives. It may be useful to mine key genes for improvement of cotton response to salt stress.
醛脱氢酶（ALDHs）是一类酶的超家族，在清除活性醛分子中发挥重要作用。在本研究中，对异源四倍体栽培棉种陆地棉（G. hirsutum）以及与其二倍体祖先亚洲棉（G. arboreum）和雷蒙德氏棉（G. raimondii）平行开展了ALDH基因超家族的全基因组综合研究。分别从二倍体和异源四倍体棉种中鉴定出了属于10个家族的30个和58个ALDH基因序列。同一家族成员间的基因结构高度保守。全基因组复制和片段重复可能是陆地棉中ALDH基因超家族扩张的主要驱动力。此外，GhALDH基因在不同组织中的表达模式多样。大多数GhALDH基因在陆地棉中受盐胁迫诱导或抑制。我们的观察揭示了二倍体棉花及其异源四倍体衍生物中ALDH基因的分子进化特性。这可能有助于挖掘关键基因以改善棉花对盐胁迫的响应。
