Cui Yupeng, Ma Jianjiang, Liu Guoyuan, Wang Nuohan, Pei Wenfeng, Wu Man, Li Xingli, Zhang Jinfa, Yu Jiwen
State Key Laboratory of Cotton Biology, Cotton Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Anyang, China.
Department of Plant and Environmental Sciences, New Mexico State University, Las Cruces, NM, United States.
Front Genet. 2019 Feb 20;10:116. doi: 10.3389/fgene.2019.00116. eCollection 2019.
Cotton is an economically important crop grown for natural fiber and seed oil production. Cottonseed oil ranks third after soybean oil and colza oil in terms of edible oilseed tonnage worldwide. Glycerol-3-phosphate acyltransferase () genes encode enzymes involved in triacylglycerol biosynthesis in plants. In the present study, 85 predicted genes were identified from the published genome data in . Among them, 14, 16, 28, and 27 homologs were identified in , and , respectively. Phylogenetic analysis revealed that a total of 108 genes from cotton, and cacao could be classified into three groups. Furthermore, through comparison, the gene structure analyses indicated that genes from the same group were highly conserved between and cotton. Segmental duplication could be the major driver for gene family expansion in the four cotton species above. Expression patterns of genes were diverse in different tissues. Most genes were induced or suppressed after salt or cold stress in Upland cotton. Eight genes were co-localized with oil and protein quantitative trait locus (QTL) regions. Thirty-two single nucleotide polymorphisms (SNPs) were detected from 12 genes, sixteen of which in nine genes were classified as synonymous, and sixteen SNPs in ten genes non-synonymous. Two SNP markers of the and genes were significantly correlated with cotton oil content in one of the three field tests. This study shed lights on the molecular evolutionary properties of genes in cotton, and provided reference for improvement of cotton response to abiotic stress and the genetic improvement of cotton oil content.
棉花是一种具有重要经济价值的作物，用于生产天然纤维和棉籽油。就全球食用油籽产量而言，棉籽油仅次于大豆油和菜籽油，位居第三。甘油-3-磷酸酰基转移酶（）基因编码参与植物三酰甘油生物合成的酶。在本研究中，从已发表的基因组数据中鉴定出85个预测的基因。其中，分别在、和中鉴定出14个、16个、28个和27个同源基因。系统发育分析表明，来自棉花、和可可的总共108个基因可分为三组。此外，通过比较，基因结构分析表明同一组中的基因在和棉花之间高度保守。片段重复可能是上述四个棉花物种中基因家族扩张的主要驱动力。基因在不同组织中的表达模式多样。陆地棉中大多数基因在盐胁迫或冷胁迫后被诱导或抑制。8个基因与油和蛋白质数量性状位点（QTL）区域共定位。从12个基因中检测到32个单核苷酸多态性（SNP），其中9个基因中的16个SNP被分类为同义突变，10个基因中的16个SNP为非同义突变。在三个田间试验之一中，和基因的两个SNP标记与棉花油含量显著相关。本研究揭示了棉花中基因的分子进化特性，为改善棉花对非生物胁迫的响应和棉花油含量的遗传改良提供了参考。
