Croen K D, Ostrove J M, Dragovic L J, Straus S E
Medical Virology Section, National Institutes of Allergy and Infectious Diseases, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9773-7. doi: 10.1073/pnas.85.24.9773.
The cellular localization and viral transcription patterns of acute and latent varicella-zoster virus (VZV) infections of human sensory nerve ganglia were studied by in situ hybridization and compared with those of latent herpes simplex virus (HSV) infection. Trigeminal and dorsal root ganglia obtained at autopsy were hybridized with 35S-labeled single-stranded RNA probes homologous to VZV or HSV fragments. We have reported that HSV persists in human sensory neurons and expresses only one family of transcripts that overlap extensively with, but are opposite in polarity to, the mRNA encoding the immediate early protein termed infected cell protein 0 (ICP0). In the present study we find that latent VZV infection involves nonneuronal cells, and multiple, but not all, VZV genes are transcribed. In contrast, during varicella both neuronal and nonneuronal cells are infected, with all regions of the VZV genome analyzed being expressed. Thus, the patterns of gene expression and cellular locations of VZV and HSV infections of human ganglia differ. The differences may underlie clinical features that distinguish these infections.
通过原位杂交研究了人感觉神经节急性和潜伏性水痘-带状疱疹病毒（VZV）感染的细胞定位和病毒转录模式，并与潜伏性单纯疱疹病毒（HSV）感染的情况进行了比较。对尸检时获得的三叉神经节和背根神经节用与VZV或HSV片段同源的35S标记单链RNA探针进行杂交。我们曾报道HSV在人感觉神经元中持续存在，并且仅表达一类转录本，这些转录本与编码即刻早期蛋白即感染细胞蛋白0（ICP0）的mRNA广泛重叠，但极性相反。在本研究中，我们发现潜伏性VZV感染涉及非神经元细胞，并且多个但并非所有VZV基因都被转录。相比之下，在水痘期间，神经元和非神经元细胞均被感染，所分析的VZV基因组所有区域均有表达。因此，人神经节中VZV和HSV感染的基因表达模式和细胞定位有所不同。这些差异可能是区分这些感染的临床特征的基础。
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