Deatly A M, Spivack J G, Lavi E, Fraser N W
Proc Natl Acad Sci U S A. 1987 May;84(10):3204-8. doi: 10.1073/pnas.84.10.3204.
Transcription of the type 1 herpes simplex virus (HSV-1) genome in trigeminal ganglia of latently infected mice was studied using in situ hybridization. Probes representative of each temporal gene class were used to determine the regions of the genome that encode the transcripts present in latently infected cells. Probes encoding HSV-1 sequences of the five immediate early genes and representative early (thymidine kinase), early-late (major capsid protein), and late (glycoprotein C) genes were used in these experiments. Of the probes tested, only those encoding the immediate early gene product infected-cell polypeptide (ICP) 0 hybridized to RNA in latently infected tissues. Probes containing the other immediate early genes (ICP4, ICP22, ICP27, and ICP47) and the representative early, early-late, and late genes did not hybridize. Two probes covering approximately equal to 30% of the HSV-1 genome and encoding over 20 early and late transcripts also did not hybridize to RNA in latently infected tissues. These results, with probes spanning greater than 60% of the HSV-1 genome, suggest that transcription of the HSV-1 genome is restricted to one region in latently infected mouse trigeminal ganglia.
利用原位杂交技术研究了潜伏感染小鼠三叉神经节中1型单纯疱疹病毒（HSV-1）基因组的转录情况。使用代表每个时间基因类别的探针来确定基因组中编码潜伏感染细胞中存在的转录本的区域。在这些实验中，使用了编码五个立即早期基因的HSV-1序列以及代表性早期（胸苷激酶）、早期晚期（主要衣壳蛋白）和晚期（糖蛋白C）基因的探针。在所测试的探针中，只有那些编码立即早期基因产物感染细胞多肽（ICP）0的探针与潜伏感染组织中的RNA杂交。包含其他立即早期基因（ICP4、ICP22、ICP27和ICP47）以及代表性早期、早期晚期和晚期基因的探针未杂交。两个覆盖约30%HSV-1基因组并编码20多个早期和晚期转录本的探针也未与潜伏感染组织中的RNA杂交。这些使用跨越HSV-1基因组60%以上的探针的结果表明，HSV-1基因组的转录仅限于潜伏感染小鼠三叉神经节中的一个区域。
