Wang Pei, Yang Junli, Yerke Aaron, Sang Shengmin
Laboratory for Functional Foods and Human Health, Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, North Carolina Research Campus, Kannapolis, NC, USA.
Mol Nutr Food Res. 2017 Jul;61(7). doi: 10.1002/mnfr.201700196. Epub 2017 Jun 1.
Exposure biomarkers used for objective estimation of whole-grain (WG) intake are essential for epidemiologic studies of WG consumption, however, up to now, no exposure biomarkers were developed for WG oat intake. This study investigates the potential of oat unique components, Avenacoside-B (AVE-B) and -A (AVE-A), as exposure biomarkers of oat intake.
An in vivo study performed in mice and an in vitro batch fecal fermentation study were used to investigate the potential metabolic routes of AVE-B and -A. Twelve healthy volunteers were recruited in the human urinary pharmacokinetic study, each participant received a single dose of oat bran as breakfast, 48 h urine samples were collected at baseline and after treatment period, and AVE-B and -A were quantified by LC-MS/MS. Deglycosylation metabolic route was identified as the major metabolic path for AVE-B and -A. Urinary AVE-B and -A concentrations increased rapidly after oat ingestion, reached their maximum excretion rates (ER ) fairly simultaneously within 5 h, then decreased gradually. And the mean eliminate half-lives (T ) for AVE-B and -A were determined as 6.22 and 4.55 h, respectively.
Oat AVE-B and -A have great potential to be used as specific exposure biomarkers to reflect oat intake.
用于客观评估全谷物（WG）摄入量的暴露生物标志物对于全谷物消费的流行病学研究至关重要，然而，截至目前，尚未开发出用于燕麦全谷物摄入量的暴露生物标志物。本研究调查了燕麦独特成分燕麦皂苷 -B（AVE-B）和 -A（AVE-A）作为燕麦摄入量暴露生物标志物的潜力。
在小鼠中进行的体内研究和体外批量粪便发酵研究用于研究AVE-B和 -A的潜在代谢途径。在人体尿液药代动力学研究中招募了12名健康志愿者，每位参与者早餐接受单剂量燕麦麸，在基线和治疗期后收集48小时尿液样本，并通过LC-MS/MS对AVE-B和 -A进行定量。去糖基化代谢途径被确定为AVE-B和 -A的主要代谢途径。摄入燕麦后，尿液中AVE-B和 -A的浓度迅速增加，在5小时内相当同时达到其最大排泄率（ER），然后逐渐下降。AVE-B和 -A的平均消除半衰期（T）分别确定为6.22小时和4.55小时。
燕麦AVE-B和 -A有很大潜力用作反映燕麦摄入量的特定暴露生物标志物。
